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A quencher-free molecular beacon design based on pyrene excimer fluorescence using pyrene-labeled UNA (unlocked
Kasper K Karlsen1, Anders Okholm, Jørgen Kjems
1Nucleic Acid Center, Department of Physics, Chemistry and Pharmacy, University of Southern Denmark, Campusvej 55, 5320 Odense M, Denmark.
Abstract:
A quencher-free molecular beacon capable of generating pyrene excimer fluorescence has been constructed using strategically positioned pyrene-UNA monomers. Hybridization of a fully complementary RNA target was accompanied by a pyrene excimer emission increase of more than 900%, and detection of RNA in living cells was demonstrated.
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