Related Experiment Video
Updated: May 11, 2026
10:17
Laboratory Protocol for Genetic Gut Content Analyses of Aquatic Macroinvertebrates Using Group-specific rDNA Primers
Published on: October 5, 2017
Permanent genetic resources added to Molecular Ecology Resources Database 1 February 2013-31 March 2013
1, M C Arias, Christiane Atteke
1Departamento de Genética e Biologia Evolutiva, Instituto de Biociências, Universidade de São Paulo, São Paulo, SP, Brazil.
Molecular Ecology Resources
|May 23, 2013
Summary
Researchers added 142 new microsatellite marker loci for 11 species, including plants and fungi, to the Molecular Ecology Resources database. These markers are valuable for population genetics and conservation studies.
Area of Science:
- Genetics
- Molecular Ecology
- Bioinformatics
Background:
- Microsatellite markers are essential tools for population genetics research.
- Expanding the number of available microsatellite loci enhances genetic diversity studies.
- The Molecular Ecology Resources database serves as a crucial repository for such genetic markers.
Purpose of the Study:
- To document the development and characterization of 142 novel microsatellite marker loci.
- To provide these new loci for 11 diverse species, including plants, fungi, and insects.
- To facilitate their inclusion in the Molecular Ecology Resources database for broader scientific use.
Main Methods:
- Development of microsatellite loci using established molecular techniques.
- Testing and validation of these loci across multiple species.
- Submission and curation of data for the Molecular Ecology Resources database.
Main Results:
- Successfully developed and characterized 142 new microsatellite marker loci.
- Loci were developed for Agriophyllum squarrosum, Amazilia cyanocephala, Batillaria attramentaria, Fungal strain CTeY1, Gadopsis marmoratus, Juniperus phoenicea subsp. turbinata, Liriomyza sativae, Lupinus polyphyllus, Metschnikowia reukaufii, Puccinia striiformis, and Xylocopa grisescens.
- Cross-testing confirmed the utility of these loci in related species, including various Amazilia hummingbirds and Liriomyza leafminers.
Conclusions:
- The addition of these 142 microsatellite loci significantly expands the available genetic resources for the studied species.
- These markers will support future research in population structure, gene flow, and conservation genetics.
- The successful cross-species amplification highlights the potential for broader application of these novel loci.
Related Concept Videos
02:39Gene Flow
Gene flow is the transfer of genes among populations, resulting from either the dispersal of gametes or from the migration of individuals.
02:51Multi-species Conserved Sequences
Next-generation sequencing technologies have created large genomic databases of a variety of animals and plants. Ever since the human genome project was completed, scientists studied the genome of primates, mammals, and other phylogenetically distant living beings. Such large-scale studies have provided new insights into the evolutionary relationship between organisms.
Although the genome of each species varies greatly from each other, a few sequences are highly conserved. Such conserved DNA...
Although the genome of each species varies greatly from each other, a few sequences are highly conserved. Such conserved DNA...
02:07Conservation of Declining Populations
Conservation of declining population focuses on ways of detecting, diagnosing, and halting a population decline. The approach uses methods to prevent populations from going extinct.
02:16Genetics of Speciation
Speciation is the evolutionary process resulting in the formation of new, distinct species—groups of reproductively isolated populations.
01:24Microbial Classification System
Classification is the process of organizing organisms into hierarchically inclusive groups based on their phenotypic similarities or evolutionary relationships. A species comprises one or more strains, and closely related species are grouped into genera. Genera are further classified into families, families into orders, orders into classes, and so forth, up to the domain level, which is the broadest taxonomic rank derived from a combination of phenotypic and genotypic data.The nomenclature of...
02:13Overview of Transposition and Recombination
Transposons make up a significant part of genomes of various organisms. Therefore, it is believed that transposition played a major evolutionary role in speciation by changing genome sizes and modifying gene expression patterns. For example, in bacteria, transposition can lead to conferring antibiotic resistance. Movement of transposable elements within the genetic pool of pathogenic bacteria can aid in transfer of antibiotic-resistant genetic elements. In eukaryotes, transposons can carry out...