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Proto-oncogenic isoform A2 of eukaryotic translation elongation factor eEF1 is a target of miR-663 and miR-744

A Vislovukh1, G Kratassiouk, E Porto

  • 1State Key Laboratory on Molecular and Cell biology, Institute of Molecular Biology and Genetics, National Academy of Sciences of Ukraine, Kiev 03680, Ukraine.

British Journal of Cancer
|May 23, 2013
PubMed
Abstract

Insights

MicroRNAs miR-663 and miR-744 inhibit the proto-oncogene eukaryotic translation elongation factor 1A2 (eEF1A2), slowing cancer cell growth. Resveratrol may boost these miRNAs, contributing to its anti-cancer effects.

Area of Science:

  • Molecular Biology
  • Oncology
  • Gene Regulation

Background:

  • Eukaryotic translation elongation factor 1A2 (eEF1A2) is identified as a proto-oncogene.
  • Cancer tissue studies suggest that loss of microRNA (miRNA)-mediated control contributes to elevated eEF1A2 expression.

Purpose of the Study:

  • To investigate the role of specific miRNAs in regulating eEF1A2 expression.
  • To explore the impact of miRNAs on cancer cell proliferation.

Main Methods:

  • Quantitative PCR (qPCR) and Western blot were used to assess miRNA effects on eEF1A2 mRNA and protein levels.
  • Dual-luciferase assays and site-directed mutagenesis of the EEF1A2 3'-UTR identified miRNA binding sites.

Main Results:

  • miR-663 and miR-744 significantly inhibited luciferase expression linked to the EEF1A2 3'-UTR.
  • Overexpression of miR-663 and miR-744 reduced eEF1A2 mRNA and protein levels in MCF7 cells.
  • Resveratrol treatment led to increased miR-663 and miR-744 levels, correlating with decreased EEF1A2 mRNA and inhibited MCF7 cell proliferation.

Conclusions:

  • miR-663 and miR-744 effectively inhibit the proto-oncogene eEF1A2, leading to reduced proliferation of MCF7 cancer cells.
  • The anti-tumor effects of resveratrol may involve the upregulation of miR-663 and miR-744.

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