Related Experiment Video
Updated: May 11, 2026

Cell Aggregation Assays to Evaluate the Binding of the Drosophila Notch with Trans-Ligands and its Inhibition by Cis-Ligands
Published on: January 2, 2018
Understanding the determinants of notch interactions with its ligands
1Centro de Biología Molecular Severo Ochoa, Consejo Superior de Investigaciones Científicas and Universidad Autónoma de Madrid, 28049 Madrid, Spain.
Abstract:
The Notch signaling pathway involves ligand-activated cleavage of the receptor Notch and the interaction of the intracellular fragment with the transcriptional regulators CSL and Mastermind. Additional complexity in the system arises through the differential interaction of Notch with its ligands of the Delta and Serrate families. Glycosylation of the extracellular portion of Notch by Fringe proteins contributes to receptor selectivity toward its ligands. Recent research suggests that a glycosylation-independent function of the Notch epidermal growth factor repeats also plays an important role in specifying activation of Notch by Ser.
Related Concept Videos
Notch Signaling Pathway
The Notch gene came into the limelight in 1914 after the discovery that its mutation in Drosophila melanogaster leads to a serrated (or "notched") wing margin phenotype. It was not until 1985...
Notch Signaling Pathway
The Notch gene came into the limelight in 1914 after the discovery that its mutation in Drosophila melanogaster leads to a serrated (or "notched") wing margin phenotype. It was not until 1985...
Role Of Notch Signalling In Intestinal Stem Cell Renewal
Direct cell-to-cell contact is needed for the activation of Notch signaling. The signal is initiated when a notch ligand binds to a receptor on an adjacent cell, also...
Ligand Binding Sites
Protein-ligand interactions are quite specific; even though numerous potential ligands surround a cellular protein at any given time, only a particular ligand can bind to that protein. Moreover, a ligand binds only to a dedicated area on the surface of the protein, known as the...
Ligand Binding Sites
Protein-ligand interactions are quite specific; even though numerous potential ligands surround a cellular protein at any given time, only a particular ligand can bind to that protein. Moreover, a ligand binds only to a dedicated area on the surface of the protein, known as the...
The Two-State Receptor Model
The binding affinity of a drug determines its interaction with one...
