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Updated: May 11, 2026

A Hydrogen-Deuterium Exchange Mass Spectrometry (HDX-MS) Platform for Investigating Peptide Biosynthetic Enzymes
Published on: May 4, 2020
A time-resolved PHIP-NMR method applied to the asymmetric homogeneous hydrogenation of β-dehydroamino acids
S Wildschütz1, P Hübler, J Bargon
1Institut für Physikalische und Theoretische Chemie der Universität Bonn, Wegelerstrasse 12, 53115 Bonn, Germany.
Abstract:
A new NMR technique based on parahydrogen-induced polarisation (PHIP) is presented, that allows the catalytic enantioselective hydrogenation of prochiral substrates to be monitored (see scheme, the left-hand side arises from the hydrogenation of a prochiral alkene). The approach takes into consideration the kinetics of hydrogenation as well as the relaxation of polarisation. The use of parahydrogen temporarily generates diastereomers which can be monitored as a function of time where otherwise only enantiomers are formed.
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