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Related Concept Videos

Bioreactor Controls-I01:28

Bioreactor Controls-I

Maintaining optimal conditions within fermenters is essential for maximizing microbial productivity and ensuring process efficiency. This lesson focuses on key parameters—temperature, foam, pH, carbon dioxide, oxygen, and pressure—and their precise measurement and control strategies in fermentation systems.Temperature ControlTemperature regulation is critical due to the exothermic nature of many fermentation processes. In small laboratory fermenters, temperature is commonly monitored using...
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Bioreactor Design and Operational System01:29

Bioreactor Design and Operational System

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In aerobic fermentations, oxygen is vital for microbial growth and metabolite production. Since air comprises only about 20% oxygen and the gas is poorly soluble in water—just 9 ppm at 20°C—supplying sufficient oxygen becomes a critical challenge, especially in high-demand processes like yeast growth or citric acid production. Even a fully saturated broth may offer only a few seconds of oxygen availability.To address this, sterile or scrubbed air is introduced into the fermentor via a sparger...
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Updated: May 11, 2026

Continuously-stirred Anaerobic Digester to Convert Organic Wastes into Biogas: System Setup and Basic Operation
11:31

Continuously-stirred Anaerobic Digester to Convert Organic Wastes into Biogas: System Setup and Basic Operation

Published on: July 13, 2012

Avoiding digester upset.

Phillip L Thompson1, Frederick W Jiencke, Shawn W Reinhart

  • 1Department of Civil & Environmental Engineering, Seattle University, 901 12th Avenue, Seattle, WA 98122, USA. thompson@seattleu.edu

Water Environment Research : a Research Publication of the Water Environment Federation
|May 24, 2013
PubMed
Summary
This summary is machine-generated.

A water displacement system effectively replaces gas chromatography for acetate uptake bioassays, enhancing anaerobic digester stability monitoring. This method offers a practical and accessible alternative for wastewater treatment facilities.

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Area of Science:

  • Environmental Science
  • Analytical Chemistry
  • Biotechnology

Background:

  • Acetate uptake bioassay (AUB) predicts anaerobic digester stability.
  • Limited availability of gas chromatography (GC) hinders AUB adoption in wastewater treatment facilities.

Purpose of the Study:

  • Evaluate a water displacement system as a GC alternative for AUB.
  • Assess the practicality and reliability of the water displacement method for digester stability prediction.

Main Methods:

  • Compared methane generation rates measured by water displacement and GC.
  • Quantified accuracy using abiotic tests with sodium carbonate and hydrochloric acid.
  • Determined method precision and method detection limit (MDL).

Main Results:

  • Methane generation rates were statistically equivalent between methods.
  • Method precision was within 5%.
  • The MDL was determined to be 0.6 mL.

Conclusions:

  • A water displacement system is a viable and accurate surrogate for GC in AUB.
  • This alternative method can increase AUB adoption for digester stability monitoring.
  • Wastewater facilities can practically implement AUB using this accessible technology.