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Published on: November 17, 2011
Calcium isotope fractionation in liquid chromatography with crown ether resin in methanol medium
Shigeki Nemoto1, Masao Nomura, Takao Oi
1Faculty of Science and Technology, Sophia University, Tokyo, Japan.
Abstract:
Breakthrough mode liquid chromatography was employed to investigate calcium (Ca) isotope fractionation in methanol medium. Highly porous silica beads, the inner pores of which were embedded with a benzo-18-crown-6 ether resin, were used as column packing material. Enrichment of heavier isotopes of Ca was observed in the frontal part of the respective Ca chromatograms. The values of the isotope fractionation coefficient (ϵ) were in the order of 10(-3) at 25 °C. Use of methanol as solvent has little advantage over the aqueous system as far as the values of ϵ are concerned. However, a substantial improvement was observed concerning the adsorption capacity of the crown ether resin for Ca ions. Molecular orbital calculations supported the present isotopic results in a qualitative fashion.
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