Related Experiment Video
Updated: May 11, 2026

Using Reverse Genetics to Manipulate the NSs Gene of the Rift Valley Fever Virus MP-12 Strain to Improve Vaccine Safety and Efficacy
Published on: November 1, 2011
Reverse genetics identifies the product of open reading frame 4 as an essential particle assembly factor of Nyamanini
Marieke Herrel1, Lars Haag, Josefina Nilsson
1Department of Virology, University of Freiburg, Freiburg, Germany.
Abstract:
We established a reverse genetics system for Nyamanini virus (NYMV) and recovered green fluorescent protein (GFP)-expressing virus from full-length cDNA. Using this technology, we assessed the functions of two poorly characterized viral genes. NYMV lacking open reading frame 2 (ORF2) could not be rescued, whereas virus lacking ORF4 was replication competent. ORF4-deficient NYMV readily established a persisting noncytolytic infection but failed to produce infectious viral particles, supporting the view that ORF4 represents an essential factor for NYMV particle assembly.
Related Concept Videos
Leaky Scanning
Retroviruses
Exon Recombination
Exon shuffling follows “splice frame rules.” Each exon has three reading...

