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A Liquid Phase Affinity Capture Assay Using Magnetic Beads to Study Protein-Protein Interaction: The Poliovirus-Nanobody Example
Published on: May 29, 2012
Development of poliovirus extraction method from stool extracts by using magnetic nanoparticles sensitized with
1Department of Virology II, National Institute of Infectious Diseases, Tokyo, Japan. minetaro@nih.go.jp
Journal of Clinical Microbiology
|May 24, 2013
Abstract:
A method for extracting poliovirus (PV) from stool extracts was developed. Magnetic nanoparticles sensitized with soluble PV receptor efficiently extracted PV pseudovirus (>99% extraction) or endogenous infectious PVs (>90% extraction) from stool extracts. This method would be useful for extraction of PV from crude biological samples.
