Oxidative status interactome map: towards novel approaches in experiment planning, data analysis, diagnostics and...

Area of Science:

• Biochemistry
• Molecular Biology
• Cellular Biophysics

Background:

• Reactive oxygen/nitrogen species (ROS/RNS) production is linked to diverse cellular processes.
• Deregulation of ROS/RNS metabolism, particularly oxidative stress, is implicated in numerous pathologies.
• Oxidative stress is an endpoint of complex dysregulated pathways, termed "oxidative status".

Purpose of the Study:

• To propose and elaborate the concept of "oxidative status" for novel diagnostic and therapeutic approaches.
• To develop a new logic system for comprehending cellular pro- and antioxidative components.
• To create a systematic, interactive interactome map of human cellular oxidative status.

Main Methods:

• Systematic literature review and data curation.
• Development of an interactive interactome map of cellular oxidative status.
• Hierarchical subdivision of over 300 factors and their interactions for logical analysis.

Main Results:

• A curated, interactive interactome map of human cellular oxidative status was developed.
• The map integrates data from over 600 selected research papers.
• It comprises more than 300 individual factors and their interactions, organized hierarchically.

Conclusions:

• The "oxidative status" concept requires a comprehensive logic system for its features and complexity.
• The developed interactome map aids in systematizing experimental data on cellular oxidative status.
• Pilot application suggests potential for developing oxidative status-based technologies.