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Related Experiment Video

Updated: May 11, 2026

A RANKL-based Osteoclast Culture Assay of Mouse Bone Marrow to Investigate the Role of mTORC1 in Osteoclast Formation

A RANKL-based Osteoclast Culture Assay of Mouse Bone Marrow to Investigate the Role of mTORC1 in Osteoclast Formation

Published on: March 15, 2018

Activated human T cells express alternative mRNA transcripts encoding a secreted form of RANKL.

N C Walsh¹, K A Alexander, C A Manning

¹St Vincent's Institute of Medical Research, Melbourne, Victoria, Australia.

Genes and Immunity

|May 24, 2013

Summary

This summary is machine-generated.

Researchers discovered a new TNFSF11 messenger RNA (mRNA) transcript variant. This variant encodes a secreted form of receptor activator of nuclear factor-kappaB-ligand (RANKL) that supports osteoclast differentiation.

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Area of Science:

Molecular Biology
Immunology
Bone Biology

Background:

Receptor activator of nuclear factor-kappaB-ligand (RANKL) is crucial for osteoclastogenesis and implicated in pathologic bone loss.
The TNFSF11 gene encodes RANKL, with known transcript variants producing membrane-bound and secreted forms.

Purpose of the Study:

To identify and characterize novel TNFSF11 transcript variants.
To investigate the expression and function of a newly identified secreted RANKL transcript.

Main Methods:

Identification of a novel TNFSF11 transcript variant.
Expression analysis in Saos-2 cells, primary human T cells, and Jurkat T cells.
In vitro functional assays to assess osteoclast differentiation support.

Main Results:

A novel TNFSF11 transcript variant extending the secreted RANKL form was identified.
This variant is expressed in osteosarcoma and T cells, with upregulated expression upon T cell activation.
The secreted RANKL produced from this transcript supports osteoclast differentiation in vitro.

Conclusions:

The TNFSF11 locus exhibits complexity, allowing for alternate mRNA transcripts encoding different RANKL isoforms.
Alternate RANKL mRNA transcripts and their protein isoforms warrant careful consideration in studies of RANKL function, especially in pathologic bone loss.