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Related Concept Videos

Resting Potential Decay01:15

Resting Potential Decay

The resting membrane potential of a neuron (-70mV) is sustained due to the selective ion permeability of the membrane. At the resting potential, the membrane is slightly permeable to ions like sodium (Na+) and chloride (Cl−) and highly permeable to potassium ions (K+). Differences in the ions' concentration inside the cell compared to the outside are maintained by membrane transport proteins like channels and pumps.
At rest, the K+ is the main ion that moves across the membrane through...
The Resting Membrane Potential01:21

The Resting Membrane Potential

Overview
Resting Membrane Potential01:24

Resting Membrane Potential

The relative difference in electrical charge, or voltage, between the inside and the outside of a cell membrane, is called the membrane potential. It is generated by differences in permeability of the membrane to various ions and the concentrations of these ions across the membrane.
The Inside of a Neuron is More Negative
The membrane potential of a cell can be measured by inserting a microelectrode into a cell and comparing the charge to a reference electrode in the extracellular fluid. The...
Potentiometry: Membrane Electrodes01:15

Potentiometry: Membrane Electrodes

Membrane electrodes, also known as p-ion electrodes, use membranes that selectively interact with free analyte ions, generating a potential difference across the membrane. The resulting membrane potential, known as the asymmetry potential, is not zero even when analyte concentrations on both sides of the membrane are equal. The membrane's response is typically not selective to a single analyte but proportional to the concentration of all ions in the sample solution capable of interacting at the...
Cellular Membranes and Drug Transport01:24

Cellular Membranes and Drug Transport

Drugs must traverse multiple biological barriers, such as multi-layered skin, single-layered intestinal epithelium, and the plasma membrane, to reach their target sites within the body. The plasma membrane, a highly structured composite of phospholipids, carbohydrates, and proteins, is the cell's protective boundary, facilitating selective substance exchange.
Phospholipids arrange themselves into a bilayer, with hydrophilic heads oriented outward and hydrophobic tails facing inward.
Mechanisms of Membrane Domain Formation00:59

Mechanisms of Membrane Domain Formation

Different physical properties of lipids and proteins allow them to localize and form distinct islands or domains in the membrane. Some membrane domains are formed due to protein-protein interactions, whereas others are formed due to the presence of specific lipids such as sphingolipids and sterols—for example, large proteins, such as bacteriorhodopsin, aggregate and create distinct domains.
Another mechanism for membrane domain formation involves membrane proteins interacting with cytoskeletal...

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Updated: May 11, 2026

Native Cell Membrane Nanoparticles System for Membrane Protein-Protein Interaction Analysis
07:31

Native Cell Membrane Nanoparticles System for Membrane Protein-Protein Interaction Analysis

Published on: July 16, 2020

Membrane potential mediates the cellular binding of nanoparticles.

Edwin H Shin1, Ye Li, Umesh Kumar

  • 1School of Chemistry and Biochemistry, Georgia Institute of Technology, 901 Atlantic Drive, Atlanta, Georgia 30332, USA.

Nanoscale
|May 24, 2013
PubMed
Summary

Cellular binding of anionic nanoparticles decreases with lower cell membrane potential. This finding is crucial for nanoparticle applications in disease detection and treatment, especially in conditions like cancer.

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Area of Science:

  • Biophysics
  • Cell Biology
  • Nanotechnology

Background:

  • Nanoparticle interactions with cells are vital for therapeutic and sensing applications.
  • Cell surface binding depends on nanoparticle properties (size, charge) and cellular factors.
  • Cells possess a transmembrane electrical potential, typically negative (-10 to -100 mV).

Purpose of the Study:

  • To investigate the impact of cell membrane potential on nanoparticle cellular binding.
  • To determine if this effect is general across different conditions.
  • To understand implications for nanoparticle-based disease diagnostics and therapeutics.

Main Methods:

  • Utilized flow cytometry and fluorescence microscopy for experimental analysis.
  • Employed dissipative particle dynamics simulations for computational modeling.
  • Examined anionic and cationic nanoparticle interactions with various cell types.

Main Results:

  • Decreased membrane potential significantly reduces anionic nanoparticle binding.
  • This effect is independent of nanoparticle composition and cell type.
  • Increased membrane potential enhances anionic nanoparticle binding.
  • Cationic nanoparticle binding is minimally affected by membrane potential.

Conclusions:

  • Cell membrane potential is a critical, tunable parameter influencing anionic nanoparticle cellular binding.
  • Understanding this relationship is essential for optimizing nanoparticle delivery and efficacy in biomedical applications.
  • The findings are particularly relevant for nanoparticle use in diseases characterized by altered membrane potential, such as cancer.