Related Experiment Video
Updated: May 11, 2026
Adapting the Electrospinning Process to Provide Three Unique Environments for a Tri-layered In Vitro Model of the Airway Wall
Published on: July 31, 2015
General protocol for the culture of cells on plasma-coated electrospun scaffolds
A Géraldine Guex1, Giuseppino Fortunato, Dirk Hegemann
1Swiss Federal Laboratories of Materials Science and Technology, St. Gallen, Switzerland.
Abstract:
As opposed to culture on standard tissue-treated plastic, cell culture on three-dimensional scaffolds impedes additional challenges with respect to substrate preparation, cell seeding, culture maintenance, and analysis. We herewith present a general route for the culture of primary cells, differentiated cells, or stem cells on plasma-coated, electrospun scaffolds. We describe a method to prepare and fix the scaffolds in culture wells and discuss a convenient method for cell seeding and subsequent analysis by scanning electron microscopy or immunohistology.
