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Related Concept Videos

Cell Specific Gene Expression01:58

Cell Specific Gene Expression

Multicellular organisms contain a variety of structurally and functionally distinct cell types, but the DNA in all the cells originated from the same parent cells. The differences in the cells can be attributed to the differential gene expression. Liver cells, whose functions include detoxification of blood, production of bile to metabolize fats, and synthesis of proteins essential for metabolism, must express a specific set of genes to perform their functions. Gene expression also varies with...

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New insights into adipocyte-specific leptin gene expression.

Christiane D Wrann1, Evan D Rosen

  • 1Division of Endocrinology, Diabetes, and Metabolism; Beth Israel Deaconess Medical Center; Boston, MA USA ; Dana-Farber Cancer Institute and Department of Cell Biology; Harvard Medical School; Boston, MA USA.

Adipocyte
|May 24, 2013
PubMed
Summary

Researchers identified the transcription factor FOSL2 as a key regulator of leptin (LEP) gene expression in fat cells. This discovery sheds light on the transcriptional pathways controlling leptin production and its physiological roles.

Keywords:
ChIP-SeqFosl2SILACadiposediabetesleptinobesitytranscription

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Area of Science:

  • Endocrinology
  • Molecular Biology
  • Genetics

Background:

  • Leptin, an adipocyte-derived hormone, regulates critical physiological functions like satiety and immunity.
  • Transcriptional pathways governing adipocyte-specific leptin (LEP) gene expression remain largely uncharacterized.

Purpose of the Study:

  • To identify cis-regulatory elements controlling adipocyte-specific LEP gene expression.
  • To discover transcription factors that bind these elements and regulate LEP expression.

Main Methods:

  • Utilized BAC transgenic reporter mice, in vitro reporter assays, and chromatin state mapping to identify a cis-element upstream of the LEP gene.
  • Employed quantitative proteomics (SILAC) with affinity enrichment to identify protein-DNA interactions.
  • Validated FOSL2's role in LEP regulation using cell culture and mouse models.

Main Results:

  • Identified an adipocyte-specific cis-regulatory element upstream of the human LEP gene.
  • Discovered that the transcription factor FOSL2 specifically binds to this region.
  • Confirmed FOSL2 as a significant regulator of LEP gene expression both in vitro and in vivo.

Conclusions:

  • FOSL2 is a key transcriptional regulator of adipocyte-specific leptin production.
  • The identified cis-element and FOSL2 provide novel insights into LEP gene transcriptional control.
  • Further research into FOSL2 and leptin in physiological and pathophysiological contexts is warranted.