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Human alpha B-crystallin (CRYA2) gene mapped to chromosome 11q12-q23
R H Brakenhoff1, A H Guerts van Kessel, M Oldenburg
1Department of Molecular Biology and Cell Biology, University of Nijmegen, The Netherlands.
Abstract:
The alpha B-crystallin gene (CRYA2) encodes the abundant lens protein alpha B-crystallin. A panel of human/rodent hybrid cell lines, derived from five different parental combinations, was characterized with respect to human chromosomal content and the presence of well-established human chromosome-specific markers. This panel was screened for the presence of CRYA2, using the third exon of the hamster alpha B-crystallin gene as a probe. The patterns of segregation of CRYA2 with individual human chromosomes show the highest degree of concordance between CRYA2 and chromosome 11. Using cell hybrids containing translocated and/or partially deleted human chromosomes, the CRYA2 gene was localized to 11q12-11q23.