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Updated: May 11, 2026

Mouse Sperm Cryopreservation and Recovery using the I·Cryo Kit
Published on: December 12, 2011
[Proteins in sperm and seminal plasma associated with human sperm resistance to cryopreservation]
Xin-Zong Zhang1, Cheng-Liang Xiong
1Zhejiang Institute of Planned Parenthood Research, Hangzhou, Zhejiang 310012, China. zxz20090722@sohu.com
Objective:
To identify the proteins that could improve the resistance of human sperm to cryopreservation using comparative proteomics.
Methods:
A total of 31 semen samples from 10 donors were divided into a high recovery and a low recovery group. Differentially expressed proteins in sperm and seminal plasma were detected and compared between the two groups by two-dimensional differential gel electrophoresis and mass spectrometry.
Results:
Totally, 22 differentially expressed proteins were found in the two groups, 12 seminal plasma proteins, 9 sperm proteins, and 1 belonging to both. These identified proteins were involved in the maturation, movement, energy metabolism, DNA repair and other activities of spermatozoa.
Conclusion:
Many proteins were identified in sperm and seminal plasma that might influence the resistance of human sperm to cryopreservation.
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