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Related Experiment Video

Updated: May 11, 2026

Combining Reflectance Confocal Microscopy with Optical Coherence Tomography for Noninvasive Diagnosis of Skin Cancers via Image Acquisition

Combining Reflectance Confocal Microscopy with Optical Coherence Tomography for Noninvasive Diagnosis of Skin Cancers via Image Acquisition

Published on: August 18, 2022

Three-dimensional visualization of dermal skin structure using confocal laser scanning microscopy.

A P M Antunes¹, A D Covington, N Petford

¹Institute for Creative Leather Technologies, University of Northampton, Northampton, UK. paula.antunes@northampton.ac.uk

Journal of Microscopy

|May 25, 2013

Summary

Researchers developed new 3-D visualization methods for skin tissue using confocal microscopy. This technique reveals detailed collagen structure, aiding in understanding material properties and medical applications.

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Generation of Self-assembled Vascularized Human Skin Equivalents

Generation of Self-assembled Vascularized Human Skin Equivalents

Published on: February 12, 2021

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Last Updated: May 11, 2026

Combining Reflectance Confocal Microscopy with Optical Coherence Tomography for Noninvasive Diagnosis of Skin Cancers via Image Acquisition

Combining Reflectance Confocal Microscopy with Optical Coherence Tomography for Noninvasive Diagnosis of Skin Cancers via Image Acquisition

Published on: August 18, 2022

Generation of Self-assembled Vascularized Human Skin Equivalents

Generation of Self-assembled Vascularized Human Skin Equivalents

Published on: February 12, 2021

Area of Science:

Biomedical Engineering
Histology
Microscopy

Background:

Collagen-based material properties are influenced by fibre bundle architecture.
Understanding dermal collagen structure is crucial for material science and medicine.

Purpose of the Study:

To develop and apply novel methods for visualizing the human dermis.
To enable three-dimensional (3-D) evaluation of collagen macromolecular structure.

Main Methods:

Utilized confocal laser scanning microscopy on thin tissue sections stained with haematoxylin and eosin.
Processed image stacks for 3-D visualization on a PC and immersive technology systems.

Main Results:

Successfully achieved 3-D visualization of dermal collagen fibre bundles.
Enabled detailed evaluation of collagen fibre pattern, orientation, and weave.

Conclusions:

The developed methods offer a novel approach to visualizing complex organic structures.
This technique has significant potential for applications in the medical field, particularly in tissue engineering and diagnostics.