Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Drug Dosing in Renal Diseases: Estimation of Glomerular Filtration Rate Based on Serum Creatinine Concentration01:28

Drug Dosing in Renal Diseases: Estimation of Glomerular Filtration Rate Based on Serum Creatinine Concentration

Glomerular filtration rate (GFR) can be estimated from serum creatinine using the modification of diet in renal disease (MDRD) formula or the chronic kidney disease–epidemiology collaboration (CKD–EPI) equation. Both methods are widely used in clinical practice to assess kidney function and guide treatment decisions.The MDRD equation does not require weight or height measurements and is normalized to the body surface area of 1.73 m², considered the average adult surface area. This equation is...
Drug Dosing in Renal Diseases: Measurement of Glomerular Filtration Rate01:25

Drug Dosing in Renal Diseases: Measurement of Glomerular Filtration Rate

The glomerular filtration rate (GFR) is a critical indicator of kidney health, reflecting how well the kidneys filter blood. Changes in GFR can signal potential kidney impairment, necessitating accurate measurement methods to monitor kidney function effectively.Various molecules can serve as markers for GFR measurement, with the ideal marker meeting several specific criteria. It must freely filter at the glomerulus, avoid reabsorption or secretion by the renal tubules, remain unmetabolized, not...
Estimation of the Physical Quantities01:05

Estimation of the Physical Quantities

On many occasions, physicists, other scientists, and engineers need to make estimates of a particular quantity. These are sometimes referred to as guesstimates, order-of-magnitude approximations, back-of-the-envelope calculations, or Fermi calculations. The physicist Enrico Fermi was famous for his ability to estimate various kinds of data with surprising precision. Estimating does not mean guessing a number or a formula at random. Instead, estimation means using prior experience and sound...
Drug Dosing in Renal Diseases: Measurement of Serum Creatinine Concentration and Clearance01:25

Drug Dosing in Renal Diseases: Measurement of Serum Creatinine Concentration and Clearance

In healthy individuals, serum creatinine levels remain stable due to a balance between its constant production—primarily from muscle metabolism—and renal excretion. Creatinine is freely filtered by the glomeruli, making it a valuable marker for estimating renal function. When the glomerular filtration rate (GFR) decreases, the kidneys can only eliminate less creatinine, causing serum levels to rise.Serum creatinine concentration is widely used to estimate creatinine clearance (Clcr), a...
One-Compartment Open Model: Wagner-Nelson and Loo Riegelman Method for ka Estimation01:24

One-Compartment Open Model: Wagner-Nelson and Loo Riegelman Method for ka Estimation

This lesson introduces two critical methods in pharmacokinetics, the Wagner-Nelson and Loo-Riegelman methods, used for estimating the absorption rate constant (ka) for drugs administered via non-intravenous routes. The Wagner-Nelson method relates ka to the plasma concentration derived from the slope of a semilog percent unabsorbed time plot. However, it is limited to drugs with one-compartment kinetics and can be impacted by factors like gastrointestinal motility or enzymatic degradation.
On...
Glomerular Filtration Rate and its Regulation01:28

Glomerular Filtration Rate and its Regulation

The Glomerular Filtration Rate (GFR) is a measure of kidney function, reflecting the volume of filtrate formed per minute in the kidneys. On average, GFR is approximately 125 mL/min in males and 105 mL/min in females. Maintaining a relatively constant GFR is essential for the kidneys to effectively regulate body fluid homeostasis and maintain extracellular stability.
GFR regulation involves two primary intrinsic controls: the myogenic and tubuloglomerular feedback mechanisms.
The myogenic...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Individualised treatment effects of corticosteroids in IgA nephropathy.

EBioMedicine·2026
Same author

Impact of Prior Adverse Pregnancy Outcome on Kidney Structure and Function in Kidney Donors.

Kidney360·2026
Same author

Kidney Stone Research in the Rochester Epidemiology Project.

Mayo Clinic proceedings·2026
Same author

Early Experience With Beta-Lactam Therapeutic Drug Monitoring in an Outpatient Parenteral Antimicrobial Therapy (OPAT) Program.

Open forum infectious diseases·2026
Same author

Accuracy and precision of automated kidney stone detection on CT.

Abdominal radiology (New York)·2026
Same author

Self-supervised Deep Learning for Denoising in Ultrasound Microvascular Imaging.

Biomedical signal processing and control·2026

Related Experiment Video

Updated: May 11, 2026

A High-throughput Method for Measurement of Glomerular Filtration Rate in Conscious Mice
07:07

A High-throughput Method for Measurement of Glomerular Filtration Rate in Conscious Mice

Published on: May 10, 2013

GFR estimating equations: getting closer to the truth?

Andrew D Rule1, Richard J Glassock

  • 1Division of Nephrology and Hypertension, Mayo Clinic, 200 First Street SW, Rochester, MN 55905, USA. rule.andrew@mayo.edu

Clinical Journal of the American Society of Nephrology : CJASN
|May 25, 2013
PubMed
Summary

Estimated glomerular filtration rate (eGFR) use in chronic kidney disease (CKD) is limited. Serum creatinine and cystatin C alone may be better for CKD diagnosis and progression than eGFR.

More Related Videos

Physiology Lab Demonstration: Glomerular Filtration Rate in a Rat
06:58

Physiology Lab Demonstration: Glomerular Filtration Rate in a Rat

Published on: July 26, 2015

Related Experiment Videos

Last Updated: May 11, 2026

A High-throughput Method for Measurement of Glomerular Filtration Rate in Conscious Mice
07:07

A High-throughput Method for Measurement of Glomerular Filtration Rate in Conscious Mice

Published on: May 10, 2013

Physiology Lab Demonstration: Glomerular Filtration Rate in a Rat
06:58

Physiology Lab Demonstration: Glomerular Filtration Rate in a Rat

Published on: July 26, 2015

Area of Science:

  • Nephrology
  • Clinical Chemistry

Background:

  • Serum creatinine and cystatin C are used to estimate glomerular filtration rate (eGFR) in chronic kidney disease (CKD).
  • Choosing the best eGFR equation involves accuracy, clinical outcome discrimination, and association with CKD risk factors, but these criteria often conflict.
  • The association with CKD risk factors is crucial but frequently overlooked.

Purpose of the Study:

  • To evaluate the limitations of eGFR in CKD patients.
  • To determine appropriate applications for eGFR versus serum creatinine and cystatin C alone in CKD management.

Main Methods:

  • Analysis of the non-GFR determinants of serum creatinine and cystatin C.
  • Evaluation of how age, sex, and race influence eGFR accuracy and interpretation.
  • Comparison of eGFR performance against measured GFR and clinical outcomes.

Main Results:

  • Non-GFR determinants and patient surrogates (age, sex, race) distort eGFR interpretation by associating with CKD risk factors and outcomes.
  • eGFR based on serum creatinine shows less bias than eGFR based on cystatin C.
  • Discrepancies exist between eGFR accuracy, outcome discrimination, and association with CKD risk factors.

Conclusions:

  • eGFR use should be restricted to specific scenarios like staging CKD severity and medication dosing.
  • Serum creatinine and cystatin C alone are more suitable for CKD diagnosis, monitoring progression, and prognostic modeling.
  • Relying solely on eGFR can lead to a distorted understanding of CKD.