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Updated: May 11, 2026

ampliPHOX Colorimetric Detection on a DNA Microarray for Influenza
Published on: June 9, 2011
Sequence-specific and visual identification of the influenza virus NS gene by azobenzene-tethered bis-peptide nucleic
Kunihiro Kaihatsu1, Shinjiro Sawada, Shota Nakamura
1The Institute of Scientific and Industrial Research, Osaka University, Osaka, Japan.
Abstract:
To rapidly and specifically identify highly virulent influenza virus strains, we prepared an azobenzene-tethered hairpin-type peptide nucleic acid, bisPNA-AZO, which has a complementary sequence against a highly conserved genomic RNA sequence within the ribonucleoprotein complex of the 2009 pandemic influenza A virus, H1N1 subtype. bisPNA-AZO recognizes the conserved virus genome sequence in a sequence-specific manner. Immobilization of bisPNA-AZO on a plate allowed capture of the target virus gene and the generation of a visual colour signal.

