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Updated: May 11, 2026

Functionalized Spirocyclic Heterocycle Synthesis and Cytotoxicity Assay
Published on: February 9, 2021
Kleinhospitines A-D, new cycloartane triterpenoid alkaloids from Kleinhovia hospita
Chang-Xin Zhou1, Li Zou, Li-She Gan
1College of Pharmaceutical Sciences, Zhejiang University, 866 Yuhangtang Road, Hangzhou 310058, PR China.
Abstract:
Kleinhospitines A-D, four unprecedented cycloartane triterpenoid alkaloids possessing a spiro α,β-unsaturated γ-lactamlactone side chain, were isolated as two mixtures of C-23 epimers from Kleinhovia hospita. Kleinhospitines C and D represent the first examples of naturally occurring cycloartane triterpenoids with a 9α,10α-cyclopropyl ring. The structures and absolute configurations were determined on the basis of spectroscopic analyses and comprehensive quantum chemical calculations. The two mixtures showed hepatoprotective activity against H2O2-induced oxidative damages on primary cultured rat hepatocytes with EC50 values of 167.0 and 126.5 μM.
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