Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Potentiometry: Membrane Electrodes01:15

Potentiometry: Membrane Electrodes

Membrane electrodes, also known as p-ion electrodes, use membranes that selectively interact with free analyte ions, generating a potential difference across the membrane. The resulting membrane potential, known as the asymmetry potential, is not zero even when analyte concentrations on both sides of the membrane are equal. The membrane's response is typically not selective to a single analyte but proportional to the concentration of all ions in the sample solution capable of interacting at the...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

The role of ATP synthase subunit e (ATP5I) in mediating the metabolic and antiproliferative effects of metformin in cancer cells.

eLife·2026
Same author

Building an oral peptide drug.

Science (New York, N.Y.)·2026
Same author

Pancreatic islet cell calcium ion imaging at single-cell resolution: functional identification of first-responder, highly connected ("hub"), and leader beta-cells.

Frontiers in endocrinology·2026
Same author

Artificial intelligence and machine learning for plasmonic and surface-enhanced sensing.

Chemical Society reviews·2026
Same author

Mitochondria-targeting symmetric diiminoguanidines: potent and selective anticancer agents against pancreatic tumors.

RSC medicinal chemistry·2026
Same author

Indigo Formation as a Predictor of Non-Native Aromatic Hydroxylation in Cytochrome P450 BM3.

ACS catalysis·2026

Related Experiment Video

Updated: May 11, 2026

Ultrasensitive Detection of Biomarkers by Using a Molecular Imprinting Based Capacitive Biosensor
08:22

Ultrasensitive Detection of Biomarkers by Using a Molecular Imprinting Based Capacitive Biosensor

Published on: February 16, 2018

Imidazolium-based ionic liquid surfaces for biosensing.

Mathieu Ratel1, Audrey Provencher-Girard, Sandy Shuo Zhao

  • 1Département de Chimie, Université de Montréal, C.P. 6128 Succ. Centre-Ville, Montreal, Quebec, Canada H3C 3J7.

Analytical Chemistry
|May 28, 2013
PubMed
Summary
This summary is machine-generated.

Ionic liquid self-assembled monolayers (SAM) offer advanced biosensing capabilities. These novel monolayers enhance streptavidin binding, improve enzyme activity, and provide low fouling for robust affinity biosensors.

More Related Videos

Biomolecular Detection employing the Interferometric Reflectance Imaging Sensor (IRIS)
11:04

Biomolecular Detection employing the Interferometric Reflectance Imaging Sensor (IRIS)

Published on: May 3, 2011

Attaching Biological Probes to Silica Optical Biosensors Using Silane Coupling Agents
09:35

Attaching Biological Probes to Silica Optical Biosensors Using Silane Coupling Agents

Published on: May 1, 2012

Related Experiment Videos

Last Updated: May 11, 2026

Ultrasensitive Detection of Biomarkers by Using a Molecular Imprinting Based Capacitive Biosensor
08:22

Ultrasensitive Detection of Biomarkers by Using a Molecular Imprinting Based Capacitive Biosensor

Published on: February 16, 2018

Biomolecular Detection employing the Interferometric Reflectance Imaging Sensor (IRIS)
11:04

Biomolecular Detection employing the Interferometric Reflectance Imaging Sensor (IRIS)

Published on: May 3, 2011

Attaching Biological Probes to Silica Optical Biosensors Using Silane Coupling Agents
09:35

Attaching Biological Probes to Silica Optical Biosensors Using Silane Coupling Agents

Published on: May 1, 2012

Area of Science:

  • Materials Science
  • Biotechnology
  • Analytical Chemistry

Background:

  • Self-assembled monolayers (SAMs) are crucial for surface functionalization in biosensors.
  • Traditional SAMs face limitations in stability, non-specific binding, and enzyme activity enhancement.
  • Ionic liquids (ILs) present a promising alternative for developing advanced SAMs.

Purpose of the Study:

  • To design and synthesize novel ionic liquid-based SAMs for enhanced biosensing applications.
  • To evaluate the performance of IL-SAMs for streptavidin binding, antibody immobilization, and enzyme activity.
  • To demonstrate the versatility of IL-SAMs in various biosensing schemes.

Main Methods:

  • Synthesis of biotinylated (IL-biotin) and carboxylated (IL-COOH) ionic liquids.
  • Self-assembly of ILs onto gold surfaces to form SAMs.
  • Surface Plasmon Resonance (SPR) spectroscopy for real-time binding analysis and enzyme activity monitoring.
  • Immobilization of antibodies and chelation of histidine-tagged proteins.

Main Results:

  • IL-biotin SAMs enabled efficient streptavidin capture and monolayer formation.
  • IL-COOH SAMs facilitated high-affinity IgG detection in the nanomolar range with low serum fouling.
  • Modified IL-COOH SAMs successfully chelated histidine-tagged proteins, enhancing enzyme activity by 24%.

Conclusions:

  • Ionic liquid self-assembled monolayers represent a versatile platform for advanced biosensing.
  • IL-SAMs demonstrate superior performance in protein binding, low non-specific binding, and enzyme activity enhancement.
  • This new class of monolayers holds significant potential for developing next-generation affinity biosensors.