Related Experiment Video
Updated: May 11, 2026
Preparation of 3D Fibrin Scaffolds for Stem Cell Culture Applications
Published on: March 2, 2012
Preparation, characterization and biological test of 3D-scaffolds based on chitosan, fibroin and hydroxyapatite for
Paulo Autran Leite Lima1, Cristiane Xavier Resende, Glória Dulce de Almeida Soares
1Departamento de Ciências de Materiais, Universidade Federal de Sergipe, São Cristóvão, Sergipe, Brazil.
Abstract:
This work describes the preparation and characterization of porous 3D-scaffolds based on chitosan (CHI), chitosan/silk fibroin (CHI/SF) and chitosan/silk fibroin/hydroxyapatite (CHI/SF/HA) by freeze drying. The biomaterials were characterized by X-ray diffraction, attenuated total reflection Fourier transform infrared spectroscopy, thermogravimetric analysis, differential scanning calorimetry, scanning electron microscopy and energy dispersive spectroscopy. In addition, studies of porosity, pore size, contact angle and biological response of SaOs-2osteoblastic cells were performed. The CHI scaffolds have a porosity of 94.2±0.9%, which is statistically higher than the one presented by CHI/SF/HA scaffolds, 89.7±2.6%. Although all scaffolds were able to promote adhesion, growth and maintenance of osteogenic differentiation of SaOs-2 cells, the new 3D-scaffold based on CHI/SF/HA showed a significantly higher cell growth at 7 days and 21 days and the level of alkaline phosphatase at 14 and 21 days was statistically superior compared to other tested materials.
