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Related Experiment Video

Updated: May 11, 2026

Fluorescent Nanoparticles for the Measurement of Ion Concentration in Biological Systems
08:17

Fluorescent Nanoparticles for the Measurement of Ion Concentration in Biological Systems

Published on: July 4, 2011

Rapid response oxygen-sensing nanofibers.

Ruipeng Xue1, Prajna Behera, Mariano S Viapiano

  • 1Department of Materials Science and Engineering, The Ohio State University, Columbus, OH 43210, USA.

Materials Science & Engineering. C, Materials for Biological Applications
|May 28, 2013
PubMed
Summary

This study developed fast-responding oxygen sensors using electrospun polycaprolactone fibers. These novel sensors offer critical metabolic insights with minimal cytotoxicity for cell viability studies.

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Area of Science:

  • Biomedical Engineering
  • Materials Science
  • Analytical Chemistry

Background:

  • Molecular oxygen is crucial for cell and tissue viability.
  • Rapid dissolved oxygen monitoring provides essential metabolic information.
  • Existing oxygen sensors often lack speed or biocompatibility for biological applications.

Purpose of the Study:

  • To develop and characterize novel, fast-response oxygen sensors using electrospun fibers.
  • To investigate the impact of fiber diameter, heat treatment, and material composition on sensor performance.
  • To assess the cytotoxicity of the developed oxygen sensors in glioblastoma cells.

Main Methods:

  • Fabrication of 0.5 μm diameter electrospun polycaprolactone (PCL) fibers containing a ruthenium(II) tris(4,7-diphenyl-1,10-phenanthroline) dichloride oxygen probe.

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  • Measurement of sensor response and recovery times, oxygen sensitivity, and photobleaching.
  • Evaluation of cytotoxicity in glioblastoma cell populations.
  • Optimization of sensor properties through heat treatment and core-shell fiber design (PES-PCL).

    • Main Results:

    • Achieved a rapid response time of 0.9±0.12 s with 0.5 μm PCL fibers.
    • Demonstrated that increased fiber diameter reduced oxygen sensitivity but minimally affected response time.
    • Heat treatment improved sensor linearity and Stern-Volmer slope.
    • Polyethersulfone (PES) or PES-PCL core-shell fibers significantly reduced photobleaching but slowed response times.
    • No apparent cytotoxicity was observed in glioblastoma cells.

    Conclusions:

    • Electrospun PCL fibers offer a promising platform for rapid dissolved oxygen sensing in biological contexts.
    • Fiber diameter and material composition can be tuned to optimize sensor speed, sensitivity, and photostability.
    • The developed sensors exhibit low cytotoxicity, making them suitable for cellular metabolic monitoring.