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Related Experiment Video

Updated: May 11, 2026

Multiplexed Single-molecule Force Proteolysis Measurements Using Magnetic Tweezers

Multiplexed Single-molecule Force Proteolysis Measurements Using Magnetic Tweezers

Published on: July 25, 2012

Single-molecule level binding force between collagen and collagen binding domain-growth factor conjugates.

Xun Huang¹, Xiaoran Li, Qiangbin Wang

¹i-LAB, Suzhou Institute of Nano-Tech and Nano-Bionics, Chinese Academy of Sciences, Suzhou, Jiangsu 215123, China.

|May 28, 2013

Summary

Collagen-binding domain (CBD) binding sites on collagen scaffolds are not uniform, influencing controlled growth factor release for tissue engineering. Understanding this interaction is key for regenerative medicine applications.

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Imaging Denatured Collagen Strands In vivo and Ex vivo via Photo-triggered Hybridization of Caged Collagen Mimetic Peptides

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Published on: January 31, 2014

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Last Updated: May 11, 2026

Multiplexed Single-molecule Force Proteolysis Measurements Using Magnetic Tweezers

Multiplexed Single-molecule Force Proteolysis Measurements Using Magnetic Tweezers

Published on: July 25, 2012

Imaging Denatured Collagen Strands In vivo and Ex vivo via Photo-triggered Hybridization of Caged Collagen Mimetic Peptides

Imaging Denatured Collagen Strands In vivo and Ex vivo via Photo-triggered Hybridization of Caged Collagen Mimetic Peptides

Published on: January 31, 2014

Area of Science:

Biomaterials Science
Tissue Engineering
Biophysics

Background:

Collagen/collagen binding domain (CBD)-growth factor composites show promise in tissue engineering and regeneration.
Fundamental biophysical questions regarding CBD binding and growth factor release mechanisms remain largely unaddressed.

Purpose of the Study:

To investigate the distribution of CBD binding sites on collagen scaffolds at the single-molecule level.
To elucidate the mechanism of controlled growth factor release from collagen scaffolds.

Main Methods:

Utilized atomic force spectroscopy (AFM)-based investigations.
Employed a quantum dot-based bimodal imaging method for visualization.
Conducted AFM force spectroscopy unbinding experiments.

Main Results:

Directly visualized non-uniformly distributed CBD binding sites on collagen membranes.
Revealed modest unbinding force and dissociation constant for the CBD/collagen interaction.

Conclusions:

The non-uniform distribution of CBD binding sites is a critical factor in controlled growth factor release.
The specific unbinding force and dissociation constant of the CBD/collagen interaction are crucial for successful controlled release applications in regenerative medicine.