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Proteomic Profile of EPS-Urine through FASP Digestion and Data-Independent Analysis
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Proteomic Profile of EPS-Urine through FASP Digestion and Data-Independent Analysis

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An in-depth comparison of the male pediatric and adult urinary proteomes

John W Froehlich1, Ali R Vaezzadeh1, Marc Kirchner2

  • 1Boston Children's Hospital, Urological Diseases Research Center, Department of Urology, Harvard Medical School, Boston, MA, USA; Proteomics Center at Boston Children's Hospital, Boston, MA, USA.

Biochimica Et Biophysica Acta
|May 28, 2013
PubMed

Insights

Urinary proteome differs significantly between infants and adults. Age-specific protein profiles highlight the need for age-matching in urinary proteomic studies for accurate biomarker discovery.

Area of Science:

  • Urology
  • Proteomics
  • Biomarker Discovery

Background:

  • The urinary proteome is a valuable source of non-invasive biomarkers.
  • Age-related changes in the urinary proteome are not well-characterized, particularly in pediatric populations.
  • Understanding these changes is crucial for accurate interpretation of proteomic data.

Purpose of the Study:

  • To characterize the male infant urinary proteome.
  • To compare the infant urinary proteome with that of healthy adults.
  • To identify age-specific and common proteins in the male urinary proteome.

Main Methods:

  • Parallel proteomic analysis of urine samples from healthy male infants (n=6) and adults (n=6).
  • Comparison with available published urinary proteomic data.
  • Gene Ontology analysis to determine functional enrichment of identified proteins.

Main Results:

  • A total of 1584 protein groups were identified.
  • 708 proteins were common to both infant and adult cohorts.
  • Infant-enriched proteins were associated with translation, transcription, growth, and metabolism.
  • Adult-enriched proteins were associated with immune response and cell adhesion.
  • Significant age-dependent quantitative differences were observed in the urinary proteome.

Conclusions:

  • The non-diseased male urinary proteome is quantitatively affected by age, exhibiting distinct age-specific subproteomes.
  • A common subproteome exists with no age-dependent abundance variations.
  • These findings underscore the critical importance of age-matching in urinary proteomic studies for reliable biomarker identification.

Keywords:
Mass spectrometryPediatric urinary proteomeSpectral countingUrinary proteomics

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