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Core-shell structured Ag@C for direct electrochemistry and hydrogen peroxide biosensor applications.

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¹College of Chemistry, Chemical Engineering and Materials Science, Soochow University, Suzhou, Jiangsu 215123, PR China.

Biosensors & Bioelectronics

|May 28, 2013

Summary

Silver-carbon (Ag@C) core-shell nanocomposites enable efficient immobilization of horseradish peroxidase (HRP) for biosensing applications. This novel material facilitates sensitive and stable detection of hydrogen peroxide (H₂O₂).

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Hydrogen Production and Utilization in a Membrane Reactor

Hydrogen Production and Utilization in a Membrane Reactor

Published on: March 10, 2023

Area of Science:

Materials Science
Nanotechnology
Electrochemistry
Biosensors

Background:

Protein immobilization is crucial for biosensor development.
Core-shell nanostructures offer unique properties for biomolecule integration.
Silver-carbon (Ag@C) nanocomposites are synthesized for advanced applications.

Purpose of the Study:

To explore Ag@C core-shell nanocomposites for protein immobilization.
To investigate the electrochemical behavior of immobilized horseradish peroxidase (HRP) on Ag@C.
To develop and evaluate a biosensor for hydrogen peroxide (H₂O₂) detection.

Main Methods:

One-step hydrothermal synthesis of Ag@C core-shell nanocomposites.
Immobilization of HRP onto Ag@C modified indium-tin-oxide (ITO) electrodes.
Electrochemical measurements (cyclic voltammetry, amperometry) and UV-vis spectroscopy.

Main Results:

Ag@C nanocomposites provided an excellent matrix for HRP adsorption, preserving bioactivity.
The HRP-Ag@C/ITO electrode demonstrated fast electron transfer and electrocatalytic reduction of H₂O₂.
The H₂O₂ biosensor exhibited a linear response from 5.0×10⁻⁷ to 1.4×10⁻⁴ M, with a detection limit of 2.0×10⁻⁷ M and a K(app)(M) of 3.75×10⁻⁵ M.

Conclusions:

The Ag@C core-shell structure is a promising material for biosensor fabrication.
The developed HRP-Ag@C/ITO bio-electrode shows high enzymatic activity, affinity, reproducibility, and stability.
Ag@C facilitates direct electrochemistry and efficient immobilization of biomolecules for biosensing.