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Cisplatin downregulates BCL2L12, a novel apoptosis-related gene, in glioblastoma cells

Mahdieh Sadat Taghavi1, Azim Akbarzadeh, Reza Mahdian

  • 1Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran. ms.taghavi@srbiau.ac.ir

In Vitro Cellular & Developmental Biology. Animal
|May 28, 2013
PubMed

Insights

Cisplatin treatment effectively reduces glioblastoma cell survival by downregulating BCL2L12 gene expression, a key factor in apoptosis resistance. This study demonstrates cisplatin

Area of Science:

  • Oncology
  • Molecular Biology
  • Cancer Research

Background:

  • Glioblastoma exhibits high apoptosis resistance and necrosis, often linked to BCL2L12 overexpression.
  • BCL2L12 inhibits caspase maturation and p53, promoting glioblastoma survival and necrosis.
  • Cisplatin is a chemotherapy agent known for inducing apoptosis.

Purpose of the Study:

  • To investigate the impact of cisplatin on BCL2L12 gene expression in glioblastoma cells.
  • To assess cisplatin's effect on glioblastoma cell viability and apoptosis induction.
  • To explore the role of BCL2L12 downregulation in cisplatin's anti-cancer activity.

Main Methods:

  • Glioblastoma cell lines were treated with varying cisplatin concentrations.
  • Cell viability and IC50 were determined using MTT assay.
  • Apoptosis was analyzed via fluorescence microscopy and flow cytometry.
  • BCL2L12 gene expression was quantified using quantitative real-time polymerase chain reaction.

Main Results:

  • Cisplatin treatment significantly reduced glioblastoma cell viability.
  • Quantitative real-time polymerase chain reaction revealed a significant downregulation of BCL2L12 gene expression (p = 0.001) following cisplatin treatment.
  • Cisplatin induced time-dependent apoptosis in glioblastoma cells.

Conclusions:

  • Cisplatin effectively induces apoptosis in glioblastoma cells.
  • The downregulation of BCL2L12 gene expression is a key mechanism by which cisplatin exerts its anti-cancer effects in glioblastoma.
  • Cisplatin represents a potential therapeutic strategy for glioblastoma, partly through modulating BCL2L12 levels.

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