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Predicting dedifferentiation in liposarcoma: a proteomic approach.

Colt M McClain1, David B Friedman, Tahar Hajri

  • 1Department of Pathology, Microbiology and Immunology, Vanderbilt University Medical Center, 1161 21st Ave. South, Nashville, TN 37232, USA.

Virchows Archiv : an International Journal of Pathology
|May 28, 2013
PubMed
Summary

Researchers investigated protein expression in liposarcoma subtypes to find predictive markers for dedifferentiation. While SELENBP1 was decreased in dedifferentiated liposarcoma, its variability prevents its use as a reliable prognostic marker.

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Area of Science:

  • Oncology
  • Proteomics
  • Molecular Pathology

Background:

  • Liposarcomas lack predictive markers for dedifferentiation, impacting treatment decisions.
  • Identifying prognostic markers could refine surgical and adjuvant therapy for liposarcoma patients.

Purpose of the Study:

  • To characterize protein expression differences between lipoma, atypical lipomatous tumor (ALT), and well-differentiated dedifferentiated liposarcoma (DDL).
  • To identify potential protein markers predictive of dedifferentiation in liposarcoma.

Main Methods:

  • Two-dimensional difference gel electrophoresis (2D-DIGE) to compare protein expression profiles.
  • Matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) for protein identification.
  • Immunohistochemistry (IHC) for validation of SELENBP1 and ALDH2 expression.

Main Results:

  • No significant protein expression differences were found between lipoma, ALT, and DDL.
  • Mitochondrial aldehyde dehydrogenase 2 (ALDH2) and selenium-binding protein-1 (SELENBP1) were significantly down-regulated in DDL compared to ALT.
  • Immunohistochemistry confirmed decreased SELENBP1 expression in DDL, but ALDH2 levels showed no significant difference.

Conclusions:

  • SELENBP1 expression is decreased in the well-differentiated component of DDL compared to ALT.
  • Variability in SELENBP1 staining patterns in liposarcoma currently precludes its use as a predictive marker for dedifferentiation.