Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Microbial Growth Media01:27

Microbial Growth Media

Microbial growth media are essential tools in microbiology, providing the nutrients and conditions necessary to cultivate and study microorganisms. These media are categorized by their composition, consistency, and functional roles, enabling researchers to investigate microbial physiology, behavior, and interactions.Types and Consistencies of Growth MediaGrowth media can be solid, liquid, or semisolid. Solid media, often agar-based, allow visible colony growth for isolation and enumeration.
Factors Influencing Microbial Growth: pH01:29

Factors Influencing Microbial Growth: pH

Microorganisms are classified as acidophiles, neutrophiles, or alkaliphiles based on their pH growth preferences, reflecting their adaptations to specific environments. Maintaining a stable intracellular pH is critical for macromolecular stability and enzymatic activity, which can be challenged by external pH variations.Neutrophiles, such as Escherichia coli, grow optimally between pH 5.5 and 8.0. These microorganisms inhabit neutral or slightly acidic environments and employ mechanisms like...
Bacterial Phylum Actinobacteria01:30

Bacterial Phylum Actinobacteria

Coryneform bacteria are gram-positive, aerobic, nonmotile rods that exhibit irregular, club-shaped, or V-shaped arrangements. Their V-shape results from snapping division, where the inner cell wall layer forms the cross-wall, while the outer layer remains intact until it ruptures on one side, causing the daughter cells to bend away.The primary genera are Corynebacterium and Arthrobacter. Corynebacterium includes diverse species, ranging from saprophytes to pathogens like Corynebacterium...
Bacterial Growth Curve01:28

Bacterial Growth Curve

The bacterial growth curve is a fundamental concept in microbiology that describes the dynamics of bacterial population growth in a closed system with controlled environmental conditions, such as temperature and nutrient availability. This curve is divided into four distinct phases: lag, log (exponential), stationary, and death phases, each reflecting a unique stage of bacterial adaptation and growth. During the lag phase, bacteria acclimate to their surroundings by synthesizing essential...
Microbes in the Production of Fermented Foods01:27

Microbes in the Production of Fermented Foods

Lactic acid bacteria (LAB) and molds are instrumental in fermenting plant-based foods to enhance preservation and ensure year-round availability. These microbial processes convert plant carbohydrates into organic acids and other metabolites that inhibit spoilage organisms and contribute to the sensory qualities of the final product.In sauerkraut production, cabbage goes through a microbial succession that starts with cocci such as Leuconostoc mesenteroides. These microbes begin fermentation by...
Stringent Response in E. coli01:23

Stringent Response in E. coli

Bacterial growth is closely tied to nutrient availability, with cells proliferating exponentially under favorable conditions and entering a stationary phase when resources become scarce. This transition is mediated by a regulatory mechanism known as the stringent response, which allows bacteria to adapt to nutrient deprivation by modulating gene expression and metabolic activity.During nutrient scarcity, intracellular amino acid levels decline. It results in the accumulation of uncharged tRNAs...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Daily cheese intake positively affects serum osteocalcin levels, vitamin K status and bone turnover markers in elderly men and women.

BMJ nutrition, prevention & health·2026
Same author

Defining epidemiological cutoff values for Brucella melitensis: an European multicentre study.

Clinical microbiology and infection : the official publication of the European Society of Clinical Microbiology and Infectious Diseases·2025
Same author

Microbiological aspects of the traditional Travnik/Vlašić cheese manufactured in Bosnia and Herzegovina.

Journal of infection in developing countries·2023
Same author

Effect on bone anabolic markers of daily cheese intake with and without vitamin K<sub>2</sub>: a randomised clinical trial.

BMJ nutrition, prevention & health·2023
Same author

Genome-assisted Identification, Purification, and Characterization of Bacteriocins.

Bio-protocol·2022
Same author

Adaptation of <i>Brucella melitensis</i> Antimicrobial Susceptibility Testing to the ISO 20776 Standard and Validation of the Method.

Microorganisms·2022
Same journal

Advancing One Health in Africa through continental early warning environmental surveillance.

Applied and environmental microbiology·2026
Same journal

Specificity and longevity of a bacterial interspecies mutual cooperation benefiting organic micropollutant biodegradation.

Applied and environmental microbiology·2026
Same journal

Coordinated regulation of trimethylamine catabolism in abundant marine bacteria.

Applied and environmental microbiology·2026
Same journal

Synergistic and individual effects of RNase E, II, and R in the regulation of <i>Escherichia coli</i> growth and metabolism.

Applied and environmental microbiology·2026
Same journal

Linking land use to the likely origins of third-generation cephalosporin-resistant Enterobacterales in freshwater.

Applied and environmental microbiology·2026
Same journal

The human gut microbe <i>Eubacterium limosum</i> utilizes flavodoxin over ferredoxin for lactate metabolism.

Applied and environmental microbiology·2026
See all related articles

Related Experiment Video

Updated: May 11, 2026

A Rapid and Specific Microplate Assay for the Determination of Intra- and Extracellular Ascorbate in Cultured Cells
11:56

A Rapid and Specific Microplate Assay for the Determination of Intra- and Extracellular Ascorbate in Cultured Cells

Published on: April 11, 2014

Enterococcus faecalis grows on ascorbic acid.

Ibrahim Mehmeti1, Margrete Solheim, Ingolf F Nes

  • 1Laboratory of Microbial Gene Technology and Food Microbiology, Department of Chemistry, Biotechnology and Food Science, Norwegian University of Life Sciences, Ås, Norway.

Applied and Environmental Microbiology
|May 28, 2013
PubMed
Summary
This summary is machine-generated.

Enterococcus faecalis can use ascorbate for fermentation. This growth is limited by amino acid availability, requiring higher amounts than glucose-based fermentation.

More Related Videos

Growing a Cystic Fibrosis-Relevant Polymicrobial Biofilm to Probe Community Phenotypes
03:53

Growing a Cystic Fibrosis-Relevant Polymicrobial Biofilm to Probe Community Phenotypes

Published on: April 19, 2024

Measuring the Effects of Bacteria on C. Elegans Behavior Using an Egg Retention Assay
08:36

Measuring the Effects of Bacteria on C. Elegans Behavior Using an Egg Retention Assay

Published on: October 22, 2013

Related Experiment Videos

Last Updated: May 11, 2026

A Rapid and Specific Microplate Assay for the Determination of Intra- and Extracellular Ascorbate in Cultured Cells
11:56

A Rapid and Specific Microplate Assay for the Determination of Intra- and Extracellular Ascorbate in Cultured Cells

Published on: April 11, 2014

Growing a Cystic Fibrosis-Relevant Polymicrobial Biofilm to Probe Community Phenotypes
03:53

Growing a Cystic Fibrosis-Relevant Polymicrobial Biofilm to Probe Community Phenotypes

Published on: April 19, 2024

Measuring the Effects of Bacteria on C. Elegans Behavior Using an Egg Retention Assay
08:36

Measuring the Effects of Bacteria on C. Elegans Behavior Using an Egg Retention Assay

Published on: October 22, 2013

Area of Science:

  • Microbiology
  • Bacterial Physiology
  • Metabolic Studies

Background:

  • Enterococcus faecalis is a common bacterium with diverse metabolic capabilities.
  • Understanding its fermentation pathways is crucial for its role in various environments.
  • Ascorbate (Vitamin C) is a potential alternative carbon source for microbial growth.

Purpose of the Study:

  • To investigate the ability of Enterococcus faecalis to utilize ascorbate as a fermentative energy source.
  • To determine the growth characteristics and limitations when using ascorbate compared to glucose.

Main Methods:

  • Culturing Enterococcus faecalis in chemically defined media with ascorbate as the sole carbon source.
  • Comparing growth yields and nutrient demands with cultures grown on glucose.
  • Analyzing amino acid requirements under different fermentation conditions.

Main Results:

  • Enterococcus faecalis demonstrated fermentative growth utilizing ascorbate.
  • Growth yield was significantly limited by the availability of essential amino acids.
  • Bacterial amino acid demand was substantially higher during ascorbate fermentation compared to glucose fermentation.

Conclusions:

  • Enterococcus faecalis possesses the metabolic machinery to ferment ascorbate.
  • Amino acid supply is a critical limiting factor for ascorbate-dependent growth in E. faecalis.
  • This finding highlights the complex nutritional requirements of E. faecalis and potential metabolic vulnerabilities.