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MiR-145 inhibits oral squamous cell carcinoma (OSCC) cell growth by targeting c-Myc and Cdk6

Yuan Shao1, Yiping Qu, Siwen Dang

  • 1Center for Translational Medicine, The First Affiliated Hospital of Xi'an Jiaotong, Xi'an 710061, the People's Republic of China. mjji0409@163.com.

Cancer Cell International
|May 29, 2013
PubMed
Abstract

Insights

MicroRNA-145 (miR-145) acts as a tumor suppressor in oral squamous cell carcinoma (OSCC) by inhibiting cell growth. Restoring miR-145 levels in OSCC may offer diagnostic and therapeutic benefits.

Area of Science:

  • Molecular Biology
  • Genetics
  • Oncology

Background:

  • MicroRNAs (miRNAs) regulate gene expression and are implicated in cancer.
  • miR-145 is a known tumor suppressor in various cancers, but its role in oral carcinogenesis is unclear.

Purpose of the Study:

  • To determine miR-145 expression levels in oral squamous cell carcinomas (OSCCs) and normal tissues.
  • To investigate the biological functions of miR-145 in OSCC.

Main Methods:

  • Reverse transcription quantitative real-time PCR (RT-qPCR) for miR-145 expression analysis.
  • Cell proliferation, colony formation, cell cycle, apoptosis, and invasion assays were performed.

Main Results:

  • miR-145 was significantly downregulated in OSCC tissues compared to normal tissues.
  • Restoring miR-145 suppressed OSCC cell proliferation, colony formation, induced G1 phase arrest, and promoted apoptosis.
  • miR-145 was found to downregulate c-Myc and Cdk6 expression in OSCC cells.

Conclusions:

  • miR-145 functions as a tumor suppressor in OSCC by targeting c-Myc and Cdk6, inhibiting cell growth.
  • Restoring miR-145 holds potential for diagnostic and therapeutic strategies in OSCC.

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