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Updated: May 11, 2026

Measurement of Poly A Tail Length from Drosophila Larva Brain and Cell Line
Published on: January 12, 2024
LARP1 specifically recognizes the 3' terminus of poly(A) mRNA
Kazuma Aoki1, Shungo Adachi, Masae Homoto
1Molecular Profiling Research Center for Drug Discovery(molprof), National Institute of Advanced Industrial Science and Technology (AIST), 2-4-7 Aomi, Koutou, Tokyo 135-0064, Japan.
Abstract:
A poly(A) tail functions in mRNA turnover and in facilitating translation as a ribonucleoprotein complex with poly(A) binding proteins (PABPs). However, factors that associate with the poly(A) tail other than PABPs have not been described. Using proteomics, we identified candidate proteins that interact to the 3' terminus of the poly(A) tail. Among these proteins, we focused on La motif-related protein 1 (LARP1) and found that LARP1 specifically recognizes the 3' termini of normal poly(A) tails. We also reveal that LARP1 stabilizes multiple mRNAs carrying 5' terminal oligopyrimidine tract (5'TOP). Our findings suggest that LARP1 may be involved in the post-transcriptional regulation of gene expression, at least in several 5'TOP mRNAs, through the binding to 3' terminus of the poly(A) tail.
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