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Updated: May 11, 2026
Hybrid De Novo Genome Assembly for the Generation of Complete Genomes of Urinary Bacteria using Short- and Long-read Sequencing Technologies
Published on: August 20, 2021
De Novo assembly and comparative analysis of the Enterococcus faecalis genome (KACC 91532) from a Korean Neonate
Jun Sang Ham1, Woori Kwak, Oun Ki Chang
1Animal Products Research and Development Division, National Institute of Animal Science, Rural Development Administration, Suwon, 441-706, Republic of Korea.
Abstract:
Using a newly constructed de novo assembly pipeline, finished genome level assembly had been conducted for the probiotic candidate strain E. faecalis KACC 91532 isolated from a stool samples of Korean neonates. Our gene prediction identified 3,061 genes in the assembled genome of the strain. Among these, nine genes were specific only for the E. faecalis KACC 91532, compared with all of the four known reference genomes (EF62, D32, V583, OG1RF). We identified genes related to phenotypic characters and detected E. faecalis KACC 91532-specific evolutionarily accelerated genes using dN/dS analysis. From these results, we found the potential risk of KACC 91532 as a useful probiotic strain and identified some candidate genetic variations that could affect the function of enzymes.
