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Different phylogenetic profile and reduced mannose-sensitive adherence capacity characterize commensal Escherichia

Forough L Nowrouzian1, Vanda Friman, Ingegerd Adlerberth

  • 1Institution for Biomedicine, Department of Infectious Disease, University of Gothenburg, Gothenburg, Sweden. forough.nowrouzian@microbio.gu.se

Microbial Pathogenesis
|May 29, 2013
PubMed

Insights

In secretory IgA (S-IgA) deficiency, gut Escherichia coli show reduced mannose-specific (MS) adherence. This is linked to specific bacterial strains and gene expression, impacting epithelial cell interactions.

Area of Science:

  • Microbiology
  • Immunology
  • Gastroenterology

Background:

  • Secretory IgA (S-IgA) is crucial for intestinal immunity, absent in IgA deficiency.
  • S-IgA carbohydrate chains mediate mannose-specific (MS) adherence of Escherichia coli (E. coli) to epithelial cells.
  • IgA deficiency (IgAd) affects commensal E. coli's MS adherence, carriage of fimH adhesin, and gene expression.

Purpose of the Study:

  • To investigate the phylogenetic characteristics of commensal E. coli in IgA deficient individuals.
  • To determine the MS adherence properties of E. coli in IgA deficient versus healthy individuals.
  • To understand the impact of IgA deficiency on E. coli-epithelial cell interactions.

Main Methods:

  • Phylogenetic analysis of commensal E. coli isolates from IgA deficient individuals.
  • Assessment of MS adherence of E. coli to epithelial cells.
  • Comparison of E. coli microbiota composition and adherence in IgA deficient and healthy subjects.

Main Results:

  • Commensal E. coli in IgA deficient individuals belong to phylogenetic group A.
  • These group A E. coli strains exhibit significantly lower MS adherence.
  • Healthy individuals harbor predominantly group B2 E. coli with strong MS adherence.

Conclusions:

  • IgA deficiency is associated with a distinct commensal E. coli microbiota dominated by low-adherence group A strains.
  • The reduced MS adherence in IgAd individuals may be linked to altered E. coli-epithelial cell interactions.
  • These findings highlight the role of S-IgA in shaping the gut microbiota's adherence properties.
Keywords:
Commensal E. coliIgA deficiencyMannose-sensitive adherencePhylogenetic groupSecretory IgA

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