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Published on: April 13, 2019
Proteomics analysis of the Flp regulon in Lactococcus lactis subsp. cremoris
1Department of Agricultural Biotechnology, Faculty of Agriculture, Kahramanmaras Sutcu Imam University, Kahramanmaras, Turkey. ismailakyol@ksu.edu.tr
Electrophoresis
|May 29, 2013
Summary
Lactococcus lactis flpA and flpB genes regulate protein expression in response to oxygen. Mutations in these transcriptional regulators altered proteins involved in stress, metabolism, and transport.
Area of Science:
- Microbiology
- Molecular Biology
- Proteomics
Background:
- The Lactococcus lactis subsp. cremoris MG1363 genome contains two flp genes, flpA and flpB.
- FlpB proteins are suggested to function as transcriptional regulators sensitive to oxygen levels.
Purpose of the Study:
- To investigate the role of flpA and flpB genes in regulating protein expression.
- To identify proteins affected by flp mutations and oxygen availability.
Main Methods:
- Creation of flp deletion mutant strains (single and double).
- Comparative proteomic analysis using 2D electrophoresis (2DE) of wild-type and mutant strains under aerobic and anaerobic conditions.
- Identification of differentially expressed proteins using MALDI-TOF MS Peptide Mass Fingerprint.
Main Results:
- Proteomic analysis quantified hundreds of protein spots under different oxygen conditions.
- Forty-five protein spots with altered intensity were identified.
- FlpA and FlpB independently and dependently regulate various proteins, affecting oxidative stress, electron transfer, metabolism, and transport pathways.
Conclusions:
- FlpA and FlpB are key regulators of protein expression in Lactococcus lactis, responding to oxygen levels.
- These regulators control a diverse set of proteins involved in fundamental cellular processes.
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