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BODIPY-tetrazine derivatives as superbright bioorthogonal turn-on probes

Jonathan C T Carlson1, Labros G Meimetis, Scott A Hilderbrand

  • 1Center for Systems Biology, Massachusetts General Hospital, 185 Cambridge Street, Boston, MA 02114, USA.

Angewandte Chemie (International Ed. in English)
|May 29, 2013
PubMed
Abstract

No abstract available in PubMed .

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Labeling DNA Probes

DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...