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Related Concept Videos

Nucleotide Excision Repair01:38

Nucleotide Excision Repair

DNA Distortion and Damage
Cells are regularly exposed to mutagens—factors in the environment that can damage DNA and generate mutations. UV radiation is one of the most common mutagens and is estimated to introduce a significant number of changes in DNA. These include bends or kinks in the structure, which can block DNA replication or transcription. If these errors are not fixed, the damage can cause mutations, which in turn can result in cancer or disease depending on which sequences are...
Nucleotide Excision Repair01:08

Nucleotide Excision Repair

Overview
Nucleotide Excision Repair01:08

Nucleotide Excision Repair

Overview
DNA Damage Can Stall the Cell Cycle02:36

DNA Damage Can Stall the Cell Cycle

In response to DNA damage, cells can pause the cell cycle to assess and repair the breaks. However, the cell must check the DNA at certain critical stages during the cell cycle. If the cell cycle pauses before DNA replication, the cells will contain twice the amount of DNA. On the other hand, if cells arrest after DNA replication but before mitosis, they will contain four times the normal amount of DNA. With a host of specialized proteins at their disposal,cells must use the right protein at...
DNA Damage can Stall the Cell Cycle02:36

DNA Damage can Stall the Cell Cycle

In response to DNA damage, cells can pause the cell cycle to assess and repair the breaks. However, the cell must check the DNA at certain critical stages during the cell cycle. If the cell cycle pauses before DNA replication, the cells will contain twice the amount of DNA. On the other hand, if cells arrest after DNA replication but before mitosis, they will contain four times the normal amount of DNA. With a host of specialized proteins at their disposal,cells must use the right protein at...
Base Excision Repair01:54

Base Excision Repair

One of the common DNA damages is the chemical alteration of single bases by alkylation, oxidation, or deamination. The altered bases cause mispairing and strand breakage during replication. This type of damage causes minimal change to the DNA double helix structure and can be repaired by the base excision repair (BER) pathways. BER corrects damaged DNA sequences by removing the damaged base and restoring the original base sequence using the complementary strand as a template.
The first step of...

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Related Experiment Video

Updated: May 11, 2026

Visualizing and Quantifying Endonuclease-Based Site-Specific DNA Damage
10:59

Visualizing and Quantifying Endonuclease-Based Site-Specific DNA Damage

Published on: August 21, 2021

Deubiquitylating enzymes and DNA damage response pathways.

Xavier Jacq1, Mark Kemp, Niall M B Martin

  • 1MISSION Therapeutics Ltd, Babraham Research Campus, Cambridge, CB22 3AT, UK. xjacq@missiontherapeutics.com

Cell Biochemistry and Biophysics
|May 29, 2013
PubMed
Summary

Targeting deubiquitylating enzymes (DUBs) in DNA damage response (DDR) pathways offers a promising strategy for cancer therapy. Inhibiting DUBs could selectively eliminate cancer cells by exploiting their reliance on specific DDR pathways.

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Characterizing DNA Repair Processes at Transient and Long-lasting Double-strand DNA Breaks by Immunofluorescence Microscopy
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Related Experiment Videos

Last Updated: May 11, 2026

Visualizing and Quantifying Endonuclease-Based Site-Specific DNA Damage
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Visualizing and Quantifying Endonuclease-Based Site-Specific DNA Damage

Published on: August 21, 2021

Characterizing DNA Repair Processes at Transient and Long-lasting Double-strand DNA Breaks by Immunofluorescence Microscopy
08:31

Characterizing DNA Repair Processes at Transient and Long-lasting Double-strand DNA Breaks by Immunofluorescence Microscopy

Published on: June 8, 2018

Visualization of DNA Repair Proteins Interaction by Immunofluorescence
07:55

Visualization of DNA Repair Proteins Interaction by Immunofluorescence

Published on: June 26, 2020

Area of Science:

  • Biochemistry
  • Molecular Biology
  • Oncology

Background:

  • Ubiquitin and ubiquitin-like modifications regulate intracellular processes.
  • These modifications are crucial for modulating DNA damage response (DDR) pathways.

Purpose of the Study:

  • To evaluate ubiquitin pathway enzymes as potential drug targets for DDR.
  • To explore the therapeutic potential of targeting deubiquitylating enzymes (DUBs) in cancer treatment.

Main Methods:

  • Review of key deubiquitylating enzymes (DUBs) involved in DDR pathways.
  • Analysis of the synthetic lethal approach for targeted cancer therapies.

Main Results:

  • Deubiquitylating enzymes (DUBs) play a critical role in DNA damage response (DDR).
  • Targeting DUBs presents a viable strategy for selective cancer therapy.

Conclusions:

  • Deubiquitylating enzymes (DUBs) are promising therapeutic targets for cancer treatment.
  • Exploiting synthetic lethality by targeting DUBs can lead to selective elimination of cancer cells.