Related Experiment Video
Updated: May 11, 2026

Generating Homo- and Heterografts Between Watermelon and Bottle Gourd for the Study of Cold-responsive MicroRNAs
Published on: November 20, 2018
[SCoT differential expression of cold resistance related genes in Dendrobium officinale under low temperature stress]
Dong-Bin Li1, Yan-Hui Gao, Jin-Ping Si
1Nurturing Station for the State Key Laboratory of Subtropical Silviculture, Zhejiang Provinical Strategic Allance for Technical Innovation in Industry of Dendrobium officinale Zhejiang Agricultural and Forestry University, Lin'an 311300, China. comeonldb@163.com
Abstract:
In order to study the molecule mechanism of the differential expression in Dendrobium officinale under low temperature, the high cold resistance germplasms were used for constructing the RNA pools. SCoT markers were used to analyze the different cDNA pools transcribed from the RNA pools. 11 transcripts derived fragments from 500 cDNA amplified bands were amplified by 64 primers, and were sorted out, cloned, sequenced and analyzed. The results showed that cDNA pools with SCoT markers could be used for differential display in D. officinale under low temperature stress. Sequence analysis indicated that the transcripts derived fragments were significantly homologous in nucleotide sequence with membrane-associated proteins, osmotic regulation protein, CBF transcriptional factor, resistance protein. One left gene segments functions were still unknown, which may be related to the cold resistant gene expression in D. officinale.
Related Concept Videos
Responses to Heat and Cold Stress
Introduction to Plant Diversity
Gene Regulation During Sporulation
Responses to Salt Stress
Other Stress Responses in Bacteria
