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Published on: December 23, 2016
Clickable tyrosine binding bifunctional linkers for preparation of DNA-protein conjugates
Dennis M Bauer1, Ishtiaq Ahmed, Antonina Vigovskaya
1Karlsruher Institute of Technology (KIT), DFG-Center for Functional Nanostructures (CFN), Wolfgang-Gaede-Str. 1a, 76131 Karlsruhe, Germany.
Abstract:
We have prepared bifunctional linkers containing clickable functional groups that enable preparation of protein-DNA conjugates through binding onto tyrosine residues. Mild conjugation strategy was demonstrated using two proteins, streptavidin(STV) and myoglobin (Mb) and it resulted in conjugates with preserved functionality of both the proteins and DNA strands. Furthermore, we show that protein-DNA conjugates can be successfully immobilized onto solid surface containing complementary DNA strands and the enzymatic activity of Mb-DNA conjugates is even higher than that of corresponding conjugates prepared through Lys binding.
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