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Updated: May 11, 2026

Post Column Derivatization Using Reaction Flow High Performance Liquid Chromatography Columns
Published on: April 26, 2016
Applications of derivatization reactions to trace organic compounds during sample preparation based on pressurized
Antonia M Carro1, Paula González, Rosa A Lorenzo
1Dpto. de Química Analítica, Nutrición y Bromatología, Facultad de Química, Universidad de Santiago de Compostela, Avda. de las Ciencias, s/n, 15782 Santiago de Compostela, Spain.
Abstract:
Pressurized liquid extraction (PLE) is an exhaustive technique used for the extraction of analytes from solid samples. Temperature, pressure, solvent type and volume, and the addition of other reagents notably influence the efficiency of the extraction. The analytical applications of this technique can be improved by coupling with appropriate derivatization reactions. The aim of this review is to discuss the recent applications of the sequential combination of PLE with derivatization and the approaches that involve simultaneous extraction and in situ derivatization. The potential of the latest developments to the trace analysis of environmental, food and biological samples is also analyzed.
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