Endometrial dye instillation: a novel approach to histopathologic evaluation of morcellated hysterectomy specimens
Teresa Tam1, Gerald Harkins, Trevor Caldwell
1Division of Urogynecology and Minimally Invasive Gynecology, Penn State Milton S. Hershey Medical Center, Penn State College of Medicine, Department of Obstetrics and Gynecology, Hershey, Pennsylvania.
Abstract:
The purpose of this prospective pilot case study was to determine whether instillation of trypan blue dye into the uterine cavity before laparoscopic hysterectomy and morcellation aids in gross identification of endometrium. The most common commercially available trypan blue stain, VisionBlue was used in this study. Instillation was performed at the beginning of the procedure using an embryo transfer catheter. A sterile solution of trypan blue, 0.5 mL, was instilled transcervically into the uterine cavities in 12 patients before laparoscopic hysterectomy with uterine morcellation. The morcellated specimens were sent for routine gross pathologic and histologic examination. It was concluded that intrauterine instillation of trypan blue stained the endometrium, thus aiding the pathologist in identification of the endometrium in morcellated uterine specimens.
