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Updated: May 11, 2026

Setup of Capillary Electrophoresis-Inductively Coupled Plasma Mass Spectrometry (CE-ICP-MS) for Quantification of Iron Redox Species (Fe(II), Fe(III))
Published on: May 4, 2020
The effect of iron and copper as an essential nutrient on mitochondrial electron transport system and lipid
Zehra Tavsan1, Hulya Ayar Kayali
1Department of Chemistry, The Graduate School of Natural and Applied Sciences, Dokuz Eylül University, 35160 Izmir, Turkey.
Abstract:
Iron and copper are essential nutrients for all living organisms as cofactors of many enzymes and play important roles in electron transport system (ETS) enzymes which have heme and iron-sulfur centers. In the present study, ETS enzymes, namely, succinate dehydrogenase (SDH) and cytochrome c oxidase (COX), activities as well as adenine nucleotides and lipid peroxidation (LPO) levels of eukaryotic model Trichoderma harzianum grown in varied concentrations of iron (0-20 mg/l) and copper (0-25 mg/l) mediums have been examined. SDH and COX activities increased up to 10 mg/l of iron. COX and SDH activities increased significantly up to 15 and 1 mg/l of copper, respectively. ATP and ADP levels showed a positive correlation with SDH activity with respect to iron-copper concentrations. The trends of AMP were similar with those of ATP and ADP for iron concentrations, while AMP levels elevated until 5 mg/l of copper. As an indicative marker of membrane damage, LPO levels increased with iron and copper concentration. In conclusion, iron and copper concentrations are of critical importance on activities of the ETS enzymes besides adenine nucleotides and LPO levels by maintenance of this metal homeostasis.
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