Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Metastasis02:30

Metastasis

Metastasis is the spread of cancer cells from the original site to distant locations in the body. Cancer cells can spread via blood vessels (hematogenous) as well as lymph vessels in the body.
Epithelial-to-Mesenchymal Transition
The epithelial-to-mesenchymal transition or EMT is a developmental process commonly observed in wound healing, embryogenesis, and cancer metastasis. EMT is induced by transforming growth factor-beta (TGF-β) or receptor tyrosine kinase (RTK) ligands, which further...
Adaptive Mechanisms in Cancer Cells02:53

Adaptive Mechanisms in Cancer Cells

Cancer cells accumulate genetic changes at an abnormally rapid rate due to the defects in the DNA repair mechanisms. From an evolutionary perspective, such genetic instability is advantageous for cancer development. Mutant cell lines accumulate a series of beneficial mutations that contribute to their progression into cancer.
Some of the advantages that cancer cells have on normal cells include - enhanced ability to divide without terminally differentiating, induce new blood vessel formation,...
Mitogens and the Cell Cycle02:38

Mitogens and the Cell Cycle

Mitogens and their receptors play a crucial role in controlling the progression of the cell cycle. However, the loss of mitogenic control over cell division leads to tumor formation. Therefore, mitogens and mitogen receptors play an important role in cancer research. For instance, the epidermal growth factor (EGF) - a type of mitogen and its transmembrane receptor (EGFR), decides the fate of the cell's proliferation. When EGF binds to EGFR, a member of the ErbB family of tyrosine kinase...
Tumor Progression02:07

Tumor Progression

Tumor progression is a phenomenon where the pre-formed tumor acquires successive mutations to become clinically more aggressive and malignant. In the 1950s, Foulds first described the stepwise progression of cancer cells through successive stages.
Colon cancer is one of the best-documented examples of tumor progression. Early mutation in the APC gene in colon cells causes a small growth on the colon wall called a polyp. With time, this polyp grows into a benign, pre-cancerous tumor. Further...
Cancer Cell Migration through Invadopodia01:35

Cancer Cell Migration through Invadopodia

Invadosome is a broad category of cell surface structures with proteolytic activity that  degrades the extracellular matrix (ECM). Invadosomes are present in normal cell types, including macrophages, endothelial cells, and neurons, as well as tumor cells. Although the macrophage podosomes and tumor cell invadopodia are classified as invadosomes, they have different structures, molecular pathways, and functions. Podosomes are short structures that last for a few minutes. However, invadopodia can...
The Tumor Microenvironment02:17

The Tumor Microenvironment

Every normal cell or tissue is embedded in a complex local environment called stroma, consisting of different cell types, a basal membrane, and blood vessels. As normal cells mutate and develop into cancer cells, their local environment also changes to allow cancer progression. The tumor microenvironment (TME) consists of a complex cellular matrix of stromal cells and the developing tumor. The cross-talk between cancer cells and surrounding stromal cells is critical to disrupt normal tissue...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Adenosine A2A and A2B receptor signaling in neurons promotes glucose and fatty acid release in the postprandial state.

bioRxiv : the preprint server for biology·2026
Same author

Regadenoson in the rehabilitation of marginal donor lungs on ex vivo lung perfusion: A blinded multicenter randomized controlled clinical trial.

JTCVS open·2025
Same author

G protein β<sub>4</sub> as a structural determinant of enhanced nucleotide exchange in the A<sub>2A</sub>AR-Gs complex.

Research square·2024
Same author

Unlocking antitumor immunity with adenosine receptor blockers.

Cancer drug resistance (Alhambra, Calif.)·2024
Same author

1,3-Dialkylxanthine Derivatives Having High Potency as Antagonists at Human A<sub>2B</sub> Adenosine Receptors.

Drug development research·2024
Same author

Improved survival of SARS COV-2-infected K18-<i>hACE2</i> mice treated with adenosine A<sub>2A</sub>R agonist.

Heliyon·2023

Related Experiment Video

Updated: May 11, 2026

Lung Tumor Cell Recruitment Assay
04:28

Lung Tumor Cell Recruitment Assay

Published on: February 26, 2019

Adenosine promotes tumor metastasis.

Joel Linden1

  • 1Division of Inflammation Biology, La Jolla Institute of Allergy and Immunology, La Jolla, CA 92037, USA. jlinden@liai.org

Science Signaling
|May 30, 2013
PubMed
Summary
This summary is machine-generated.

Adenosine signaling reduces Rap1B prenylation and plasma membrane localization via protein kinase A (PKA) in tumor cells. This promotes cell scattering and invasiveness, key steps in tumor metastasis.

More Related Videos

Tracking Tumor Cell Dissemination from Lung Metastases Using Photoconversion
05:23

Tracking Tumor Cell Dissemination from Lung Metastases Using Photoconversion

Published on: July 7, 2023

A Preclinical Murine Model of Hepatic Metastases
06:51

A Preclinical Murine Model of Hepatic Metastases

Published on: September 27, 2014

Related Experiment Videos

Last Updated: May 11, 2026

Lung Tumor Cell Recruitment Assay
04:28

Lung Tumor Cell Recruitment Assay

Published on: February 26, 2019

Tracking Tumor Cell Dissemination from Lung Metastases Using Photoconversion
05:23

Tracking Tumor Cell Dissemination from Lung Metastases Using Photoconversion

Published on: July 7, 2023

A Preclinical Murine Model of Hepatic Metastases
06:51

A Preclinical Murine Model of Hepatic Metastases

Published on: September 27, 2014

Area of Science:

  • Molecular Biology
  • Cancer Research
  • Cell Biology

Background:

  • Tumor metastasis involves reduced cell-cell adhesion and increased cell scattering.
  • The small GTPase Rap1B stabilizes cell-cell contacts by accumulating in the plasma membrane.
  • Rap1B membrane localization is regulated by C-terminal prenylation.

Purpose of the Study:

  • To investigate the effect of protein kinase A (PKA) on Rap1B prenylation and plasma membrane localization.
  • To explore the role of adenosine signaling in regulating Rap1B function in tumor cells.

Main Methods:

  • Investigated Rap1B phosphorylation by PKA.
  • Assessed Rap1B prenylation and plasma membrane localization.
  • Examined the impact of adenosine A(2B) receptor signaling.

Main Results:

  • PKA-mediated phosphorylation of Rap1B reduced its prenylation and plasma membrane localization.
  • Persistent PKA signaling, induced by adenosine, decreased Rap1B prenylation.
  • Reduced Rap1B prenylation correlated with enhanced tumor cell scattering and invasiveness.

Conclusions:

  • Adenosine signaling negatively regulates Rap1B prenylation and membrane localization through PKA.
  • This mechanism enhances tumor cell scattering and invasiveness, contributing to metastasis.
  • Targeting adenosine signaling may offer therapeutic strategies for reducing tumor spread.