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Expression of huCdc7 in colorectal cancer
Hai-Jun Chen1, Zhen Zhu, Xue-Lin Wang
1Department of Oncology, Kunshan Hospital of Traditional Chinese Medicine Affiliated to Nanjing University of Chinese Medicine, Kunshan 215300, Jiangsu Province, China. ksschj@126.com
Aim:
To detect the expression of huCdc7 in colorectal cancer.
Methods:
The mRNA and protein expression of huCdc7 in 39 colorectal cancer tissue specimens and matched tumor-adjacent normal colorectal tissue specimens was detected by reverse transcription-polymerase chain reaction and immunohistochemistry, respectively.
Results:
The relative expression level of huCdc7 mRNA in colorectal cancer was significantly higher than that in tumor-adjacent normal colorectal tissues (0.03675 ± 1.00 vs 0.01199 ± 0.44, P < 0.05). huCdc7-positive cells displayed brown granules in the nucleus. Tumor tissues contained many huCdc7-positive cells, whereas normal colorectal tissues contained very few positive cells.
Conclusion:
huCdc7 may play an important role in the development and progression of colorectal cancer.
