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Updated: May 11, 2026
11:46
Direct Lineage Reprogramming of Adult Mouse Fibroblast to Erythroid Progenitors
Published on: December 14, 2018
Eafs control erythroid cell fate by regulating c-myb expression through Wnt signaling
1College of Fisheries, Huazhong Agricultural University, Wuhan, P. R. China.
Plos One
|May 30, 2013
Summary
EAF1/2 factors regulate erythroid cell development by controlling c-Myb expression via canonical Wnt signaling. Knockdown blocked erythroid differentiation, which was rescued by c-Myb restoration.
Area of Science:
- Developmental Biology
- Hematopoiesis
- Molecular Biology
Background:
- ELL associated factor 1 and 2 (EAF1/2) are crucial for embryogenesis and tumor suppression.
- Previous work linked EAF factors to embryonic patterning via Wnt signaling.
- The specific role of EAF factors in erythroid cell differentiation remained unclear.
Purpose of the Study:
- To investigate the role of EAF1/2 factors in erythroid cell differentiation.
- To elucidate the molecular mechanisms by which EAF factors regulate erythroid development.
Main Methods:
- Knockdown of eaf1 and eaf2 genes in zebrafish embryos.
- Morpholino-mediated knockdown of c-myb.
- Overexpression of dominant-negative Tcf (dn-Tcf) to inhibit Wnt signaling.
- Analysis of erythroid-specific gene expression (βe3 globin) and hematopoietic progenitor markers.
Main Results:
- Knockdown of EAF1/2 blocked primary erythroid differentiation while maintaining hematopoietic precursors.
- Co-injection of c-myb morpholino rescued erythroid differentiation in EAF-deficient embryos.
- Inhibition of Wnt signaling by dn-Tcf normalized c-Myb levels and restored erythroid differentiation in EAF morphants.
Conclusions:
- EAF factors control erythroid cell fate.
- This regulation occurs through modulating c-Myb expression via canonical Wnt signaling.
- EAF factors represent a novel regulatory node in erythropoiesis.
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