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Updated: May 11, 2026

A Straightforward Method for Glucosinolate Extraction and Analysis with High-pressure Liquid Chromatography (HPLC)
Published on: March 15, 2017
[HPLC characteristic chromatographic profile of Sarcandra glabra]
Pei-Ling Lin1, Jian-Wei Zeng, Chun-Hua Ding
1College of Pharmacy, Fujian University of Traditional Chinese Medicine, Fuzhou 350122, China.
Objective:
To develop the characteristic chromatographic profile of Sarcandra glabra by HPLC for its quality control.
Method:
The HPLC analysis was performed on an Agilent Zorbax Eclipse XDB-C18 column (4. 6 mm x 250 mm, 5 microm) with column temperature at 40 degree C. The mobile phase was consisted of water containing 0. 5% formic acid and acetonitrile to methanol (1:9) in gradient mode, and the detection wavelength was set at 344 nm.
Result:
A common mode of the HPLC characteristic chromatographic profile has been establised. There were 20 common peaks , seven of which were identified, and 46 samples from different habitats were classified into five groups based on principal component cluster analysis.
Conclusion:
The method was time-saving and can represent the chemical information and provide a scientific basis for quality control of S. glabra.
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