Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Transcription01:10

Transcription

Overview
Transcription is the process of synthesizing RNA from a DNA sequence by RNA polymerase. It is the first step in producing a protein from a gene sequence. Additionally, many other proteins and regulatory sequences are involved in the proper synthesis of messenger RNA (mRNA). Regulation of transcription is responsible for the differentiation of all the different types of cells and often for the proper cellular response to environmental signals.
Transcription Can Produce Different Kinds...
Responses to Heat and Cold Stress02:45

Responses to Heat and Cold Stress

Every organism has an optimum temperature range within which healthy growth and physiological functioning can occur. At the ends of this range, there will be a minimum and maximum temperature that interrupt biological processes.
Riboswitches01:56

Riboswitches

Riboswitches are non-coding mRNA domains that regulate the transcription and translation of downstream genes without the help of proteins. Riboswitches bind directly to a metabolite and can form unique stem-loop or hairpin structures in response to the amount of the metabolite present. They have two distinct regions – a metabolite-binding aptamer and an expression platform.
The aptamer has high specificity for a particular metabolite which allows riboswitches to specifically regulate...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Disentangling the importance of microbiological and physico-chemical properties of Ethiopian field soils for the Striga seed bank and sorghum infestation.

Environmental microbiome·2026
Same author

The MassBank contributions of the mFam collaboration.

Metabolomics : Official journal of the Metabolomic Society·2026
Same author

Correction to "Multi-Laboratory Assessment Reveals Variable Ion Species Profiles in Electrospray Ionization Mass Spectrometer".

Journal of the American Society for Mass Spectrometry·2026
Same author

Untargeted Metabolomics Reveals Major Patterns of Metabolic Shifts in Potato Seed Tubers during Storage.

Potato research·2026
Same author

Root traits correlate with crop rhizosphere microbiome diversity independent of legume relatedness.

ISME communications·2026
Same author

Multi-Laboratory Assessment Reveals Variable Ion Species Profiles in Electrospray Ionization Mass Spectrometry.

Journal of the American Society for Mass Spectrometry·2026

Related Experiment Video

Updated: May 11, 2026

Tomato Root Transformation Followed by Inoculation with Ralstonia Solanacearum for Straightforward Genetic Analysis of Bacterial Wilt Disease
09:05

Tomato Root Transformation Followed by Inoculation with Ralstonia Solanacearum for Straightforward Genetic Analysis of Bacterial Wilt Disease

Published on: March 11, 2020

System-wide hypersensitive response-associated transcriptome and metabolome reprogramming in tomato.

Desalegn W Etalo1, Iris J E Stulemeijer, H Peter van Esse

  • 1Laboratory of Plant Physiology, Wageningen University, 6708 PB Wageningen, The Netherlands.

Plant Physiology
|May 31, 2013
PubMed
Summary

Plant resistance involves a hypersensitive response (HR), a highly energy-demanding process. This study reveals WRKY transcription factors orchestrate HR by reprogramming plant metabolism and gene expression in tomato plants.

More Related Videos

Profiling Thiol Redox Proteome Using Isotope Tagging Mass Spectrometry
12:07

Profiling Thiol Redox Proteome Using Isotope Tagging Mass Spectrometry

Published on: March 24, 2012

High-Throughput Identification of Resistance to Pseudomonas syringae pv. Tomato in Tomato using Seedling Flood Assay
06:41

High-Throughput Identification of Resistance to Pseudomonas syringae pv. Tomato in Tomato using Seedling Flood Assay

Published on: March 10, 2020

Related Experiment Videos

Last Updated: May 11, 2026

Tomato Root Transformation Followed by Inoculation with Ralstonia Solanacearum for Straightforward Genetic Analysis of Bacterial Wilt Disease
09:05

Tomato Root Transformation Followed by Inoculation with Ralstonia Solanacearum for Straightforward Genetic Analysis of Bacterial Wilt Disease

Published on: March 11, 2020

Profiling Thiol Redox Proteome Using Isotope Tagging Mass Spectrometry
12:07

Profiling Thiol Redox Proteome Using Isotope Tagging Mass Spectrometry

Published on: March 24, 2012

High-Throughput Identification of Resistance to Pseudomonas syringae pv. Tomato in Tomato using Seedling Flood Assay
06:41

High-Throughput Identification of Resistance to Pseudomonas syringae pv. Tomato in Tomato using Seedling Flood Assay

Published on: March 10, 2020

Area of Science:

  • Plant pathology
  • Molecular biology
  • Plant-microbe interactions

Background:

  • The hypersensitive response (HR) is a critical plant defense mechanism against pathogens.
  • Understanding the molecular and metabolic changes during HR is crucial for crop improvement.

Purpose of the Study:

  • To investigate the transcriptome and metabolome reprogramming associated with HR in tomato (Solanum lycopersicum).
  • To identify key regulators and metabolic pathways involved in the HR.

Main Methods:

  • Utilized tomato plants expressing a resistance gene and the matching avirulence gene of Cladosporium fulvum.
  • Analyzed transcriptomic and metabolomic data to identify reprogramming events.
  • Examined promoter regions for transcription factor binding sites, focusing on WRKY motifs.

Main Results:

  • HR is an energy-intensive process involving ubiquitin-dependent protein degradation, sugar hydrolysis, and lipid catabolism.
  • Significant accumulation of secondary metabolites, including hydroxycinnamic acid amides, was observed.
  • Co-regulated expression of WRKY transcription factors and HR-related genes, with enrichment of W-box motifs, implicates WRKYs in HR regulation.

Conclusions:

  • WRKY transcription factors are key orchestrators of the hypersensitive response in tomato.
  • The study identified novel HR-related genes and highlighted the metabolic costs and strategies associated with plant defense.
  • Reverse genetics approaches will be essential for elucidating the function of individual HR components.