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PCR amplification and sequence analysis of GC-rich sequences: Aristaless-related homeobox example
May H Tan1, Jozef Gécz, Cheryl Shoubridge
1Department of Paediatrics, University of Adelaide, Adelaide, SA, Australia.
Abstract:
PCR amplification (followed by mutation scanning or direct sequencing) is a technique widely used in mutation detection and molecular studies of disease-causing genes, such as ARX. PCR amplification of high GC-rich regions encounters difficulties using conventional PCR procedures. Here, we present the strategies to amplify and sequence these GC-rich regions for the purposes of mutation screening and other molecular analyses.
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