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Challenges of "sticky" co-immunoprecipitation: polyalanine tract protein-protein interactions

T R Mattiske1, May H Tan, Jozef Gécz

  • 1Department of Paediatrics, University of Adelaide, Adelaide, SA, Australia.

Methods in Molecular Biology (Clifton, N.J.)
|May 31, 2013
PubMed

Co-immunoprecipitation (Co-IP) (followed by immunoblotting) is a technique widely used to characterize specific protein-protein interactions. Investigating interactions of proteins containing "sticky" polyalanine (PolyA) tracts encounters difficulties using conventional Co-IP procedures. Here, we present strategies to specifically capture proteins containing these difficult PolyA tracts, enabling subsequent robust detection of interacting proteins by Co-IP.

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