Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Video

Updated: May 11, 2026

Growth Assays to Assess Polyglutamine Toxicity in Yeast
09:06

Growth Assays to Assess Polyglutamine Toxicity in Yeast

Published on: March 5, 2012

Yeast as a platform to explore polyglutamine toxicity and aggregation.

Martin L Duennwald1

  • 1Department of Pathology, Schulich School of Medicine and Dentistry, University of Western Ontario, Ontario, Canada.

Methods in Molecular Biology (Clifton, N.J.)
|May 31, 2013
PubMed
Summary

Baker's yeast models protein misfolding diseases like Huntington's. Yeast studies identify cellular factors affecting polyglutamine toxicity and aggregation, offering insights relevant to human pathology.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

STIP1/HOP promotes the formation of cytotoxic α-synuclein oligomers.

Molecular neurodegeneration advances·2026
Same author

Investigating substrate specificity in arogenate versus prephenate dehydratases.

BMC biology·2026
Same author

FUS and TDP-43 aggregation are uncoupled from toxicity in ageing yeast models.

BMC biology·2026
Same author

Specific branches of the proteostasis network regulate the toxicity associated with mistranslation.

Nucleic acids research·2025
Same author

Stress-inducible phosphoprotein 1 (Sti1/Stip1/Hop) sequesters misfolded proteins during stress.

The FEBS journal·2024
Same author

Prion protein regulates invasiveness in glioblastoma stem cells.

BMC cancer·2024

Area of Science:

  • Molecular biology
  • Neuroscience
  • Genetics

Background:

  • Protein misfolding is implicated in neurodegenerative diseases.
  • Polyglutamine expansion proteins cause diseases like Huntington's.
  • Baker's yeast (Saccharomyces cerevisiae) is a model organism for studying cellular mechanisms.

Purpose of the Study:

  • To investigate polyglutamine toxicity and aggregation in yeast.
  • To identify cellular factors modulating polyglutamine protein behavior.
  • To establish reliable experimental protocols for yeast-based studies.

Main Methods:

  • Utilizing baker's yeast (Saccharomyces cerevisiae) as a model system.
  • Developing and applying experimental protocols to assess polyglutamine toxicity.

More Related Videos

Effect of Fluorescent Proteins on Fusion Partners Using Polyglutamine Toxicity Assays in Yeast
09:23

Effect of Fluorescent Proteins on Fusion Partners Using Polyglutamine Toxicity Assays in Yeast

Published on: November 28, 2018

A Method to Study α-Synuclein Toxicity and Aggregation Using a Humanized Yeast Model
08:24

A Method to Study α-Synuclein Toxicity and Aggregation Using a Humanized Yeast Model

Published on: November 25, 2022

Related Experiment Videos

Last Updated: May 11, 2026

Growth Assays to Assess Polyglutamine Toxicity in Yeast
09:06

Growth Assays to Assess Polyglutamine Toxicity in Yeast

Published on: March 5, 2012

Effect of Fluorescent Proteins on Fusion Partners Using Polyglutamine Toxicity Assays in Yeast
09:23

Effect of Fluorescent Proteins on Fusion Partners Using Polyglutamine Toxicity Assays in Yeast

Published on: November 28, 2018

A Method to Study α-Synuclein Toxicity and Aggregation Using a Humanized Yeast Model
08:24

A Method to Study α-Synuclein Toxicity and Aggregation Using a Humanized Yeast Model

Published on: November 25, 2022

  • Employing methods to quantify polyglutamine aggregation in yeast cells.

    • Main Results:

    • Yeast has provided fundamental insights into protein misfolding mechanisms.
    • Specific cellular factors influencing polyglutamine toxicity and aggregation were identified.
    • Findings in yeast demonstrate high relevance to other model organisms and human diseases.

    Conclusions:

    • Baker's yeast is a valuable tool for studying polyglutamine expansion protein disorders.
    • Yeast-based research contributes significantly to understanding neurodegenerative disease mechanisms.
    • The established protocols enable reliable assessment of polyglutamine toxicity and aggregation.