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Updated: May 11, 2026

High-throughput and Comprehensive Drug Surveillance Using Multisegment Injection-Capillary Electrophoresis-Mass Spectrometry
Published on: April 23, 2019
Applications of high-resolution MS in bioanalysis.
Mike-Qingtao Huang1, Zhongping John Lin, Naidong Weng
1Janssen Research & Development, LLC, Pharmaceutical Companies of Johnson & Johnson, 1000 Route 202 South, Raritan, NJ 08869, USA. qhuang5@its.jnj.com
High-resolution LC-MS (LC-HRMS) offers rapid, reliable bioanalysis and cost savings. Proper utilization ensures its significant role in quantitative bioanalysis, despite potential pitfalls.
Area of Science:
- Analytical Chemistry
- Pharmaceutical Science
Background:
- High-resolution mass spectrometry (HRMS) coupled with liquid chromatography (LC-HRMS) is increasingly accessible in pharmaceutical labs.
- Its perceived specificity, quantitation, and structural elucidation capabilities offer advantages.
Purpose of the Study:
- To share perspectives on current and future applications of LC-HRMS in bioanalysis.
- To discuss factors influencing method establishment and potential pitfalls in regulated bioanalysis.
Main Methods:
- Review of current and emerging applications of LC-HRMS in bioanalysis.
- Discussion of best practices and challenges for method development and validation.
Main Results:
- LC-HRMS enables rapid and reliable method development and sample analysis.
- Potential for cost and resource savings through efficient data processing and mining.
Conclusions:
- Appropriate utilization of LC-HRMS can lead to significant advancements in quantitative bioanalysis.
- Addressing method establishment factors and potential pitfalls is crucial for success in regulated environments.
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